Chen Yue, Yan Guo-Wei, Zheng Yue-Mei, Zhou Kai-Xuan, Xie Cui-Liu
Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University. Luzhou 646000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Feb;31(1):48-53.
To explore the effects of carbodiimide combined with different bonding techniques on the property of dentin bonding.
64 third molars in vitro were randomly divided into 8 groups(n=8), including EWB and EWBa (no pretreatment+ethanol-wet bonding), E-EWB and E-EWBa (0.5 mol/L EDC+ethanol-wet bonding), E-WB and E-WBa (0.5 mol/L EDC+water-wet bonding), E-DB and E-Dba (0.5 mol/L EDC+dry bonding). Group EWB, E-EWB, E-WB and E-DB were immediate groups, while group EWBa, E-EWBa, E-WBa and E-DBa were aging groups. Six specimens in each group were randomly chosen to test shear bonding strength and then observe failure patterns. The remaining specimens were used to observe bonding interface under SEM. SPSS 20.0 software package was used for statistical analysis of the data.
There was no significant difference in shear bonding strength between group EWB and E-EWB, group E-EWB and E-WB(P＞0.05), while shear bounding strength in group E-EWB and E-WB were significantly greater than group E-DB(P＜0.05). The shear bonding strength of group E-EWBa was significantly greater than group EWBa (P＜0.05). The shear bonding strength of group E-EWBa, E-WBa and E-DBa was gradually decreased, and their differences were significant (P＜0.05). The factors of EDC, bonding techniques and aging played a significant role in shear bonding strength (P＜0.05). Interactions between EDC and aging was significant (P＜0.05), and that between bonding techniques and aging was also significant (P＜0.05). The main failure modes of the water-wet bonding and the ethanol-wet bonding were resin failure, adhesive interface failure and mixed failure. The main failure of dry bonding was adhesive interface failure. The thickest and densest hybrid layer were found in group E-EWB. Some tiny cracks were presented between the hybrid layer and adhesives layer in group EWBa and E-EWBa. Partially uneven cracks existed in the hybrid layer in group E-WBa, and that was more obvious in group E-DBa.
EDC pretreatment combined with ethanol-wet adhesive technique acquires the optimal shear bonding strength and the durability of dentin bonding.
探讨碳二亚胺结合不同粘结技术对牙本质粘结性能的影响。
将64颗离体第三磨牙随机分为8组（n = 8），包括EWB组和EWBa组（无预处理+乙醇湿粘结）、E-EWB组和E-EWBa组（0.5 mol/L EDC+乙醇湿粘结）、E-WB组和E-WBa组（0.5 mol/L EDC+水湿粘结）、E-DB组和E-Dba组（0.5 mol/L EDC+干粘结）。EWB组、E-EWB组、E-WB组和E-DB组为即时组，而EWBa组、E-EWBa组、E-WBa组和E-Dba组为老化组。每组随机选取6个标本测试剪切粘结强度，然后观察失败模式。其余标本用于扫描电镜下观察粘结界面。使用SPSS 20.0软件包对数据进行统计分析。
EWB组与E-EWB组、E-EWB组与E-WB组之间的剪切粘结强度无显著差异（P＞0.05），而E-EWB组和E-WB组的剪切粘结强度显著高于E-DB组（P＜0.05）。E-EWBa组的剪切粘结强度显著高于EWBa组（P＜0.05）。E-EWBa组、E-WBa组和E-DBa组的剪切粘结强度逐渐降低，差异有统计学意义（P＜0.05）。EDC、粘结技术和老化因素对剪切粘结强度有显著影响（P＜0.05）。EDC与老化之间的相互作用显著（P＜0.05），粘结技术与老化之间的相互作用也显著（P＜0.05）。水湿粘结和乙醇湿粘结的主要失败模式为树脂破坏、粘结界面破坏和混合破坏。干粘结的主要失败模式为粘结界面破坏。在E-EWB组中发现最厚且最致密的混合层。在EWBa组和E-EWBa组的混合层与粘结剂层之间出现一些微小裂纹。在E-WBa组的混合层中存在部分不均匀裂纹，在E-Dba组中更明显。
EDC预处理结合乙醇湿粘结技术可获得最佳的剪切粘结强度和牙本质粘结耐久性。
