Institut Jacques Monod, CNRS, Université de Paris Cité, France.
FEBS J. 2023 Aug;290(15):3723-3736. doi: 10.1111/febs.16530. Epub 2022 Jun 5.
Transcriptome-wide interrogation of eukaryotic genomes has unveiled the pervasive nature of RNA polymerase II transcription. Virtually, any DNA region with an accessible chromatin structure can be transcribed, resulting in a mass production of noncoding RNAs (ncRNAs) with the potential of interfering with gene expression programs. Budding yeast has proved to be a powerful model organism to understand the mechanisms at play to control pervasive transcription and overcome the risks of hazardous disruption of cellular functions. In this review, we focus on the actors and strategies yeasts employ to govern ncRNA production, and we discuss recent findings highlighting the dangers of losing control over pervasive transcription.
真核生物基因组的转录组全基因组分析揭示了 RNA 聚合酶 II 转录的普遍存在。实际上，任何具有可及染色质结构的 DNA 区域都可以被转录，从而产生大量具有干扰基因表达程序潜力的非编码 RNA（ncRNA）。 budding yeast 已被证明是一种强大的模式生物，可以帮助我们理解控制普遍转录和克服细胞功能危险中断风险的机制。在这篇综述中，我们重点介绍了酵母控制 ncRNA 产生所采用的因子和策略，并讨论了最近的发现，这些发现强调了失去对普遍转录控制的危险。
