大肠杆菌中碱基替换热点的分子基础。
Molecular basis of base substitution hotspots in Escherichia coli.
作者信息
Coulondre C, Miller J H, Farabaugh P J, Gilbert W
出版信息
Nature. 1978 Aug 24;274(5673):775-80. doi: 10.1038/274775a0.
DOI:10.1038/274775a0
PMID:355893
Abstract
In the lacI gene of Escherichia coli spontaneous base substituion hotspots occur at 5-methylcytosine residues. The hotspots disappear when the respective cytosines are not methylated. We suggest that the hotspots may result from the spontaneous deamination of 5-methylcytosine to thymine, which is not excised by the enzyme DNA-uracil glycosidase.
摘要
在大肠杆菌的lacI基因中，自发碱基取代热点出现在5-甲基胞嘧啶残基处。当相应的胞嘧啶未被甲基化时，这些热点就会消失。我们认为，这些热点可能是由5-甲基胞嘧啶自发脱氨基形成胸腺嘧啶所致，而胸腺嘧啶不会被DNA尿嘧啶糖苷酶切除。
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