Ting Bernice Yii Shu, Fuloria Neeraj Kumar, Subrimanyan Vetriselvan, Bajaj Sakshi, Chinni Suresh V, Reddy Lebaka Veeranjaneya, Sathasivam Kathiresan V, Karupiah Sundram, Malviya Rishabha, Meenakshi Dhanalekshmi Unnikrishnan, Paliwal Neeraj, Priya Krishna, Fuloria Shivkanya
Faculty of Pharmacy, AIMST University, Bedong 08100, Kedah, Malaysia.
Faculty of Medicine, Bioscience and Nursing, MAHSA University, Kuala Lumpur 42610, Selangor, Malaysia.
Materials (Basel). 2022 Apr 27;15(9):3170. doi: 10.3390/ma15093170.
Periimplantitis due to pathogenic bacteria is considered as a major cause for dental implants failures. Biogenic zinc oxide nanoparticles (ZnPs) are known to inhibit periimplantitis triggering pathogens. The current investigation intended to perform ZnPs biosynthesis and evaluation against periimplantitis triggering bacteria. The current study involved ZnPs biosynthesis using leaves aqueous extract (APLAE), followed by optimization, stability, characterization, and in vitro evaluation against periimplantitis triggering bacteria. The experimental results indicated the success of ZnPs biosynthesis based on the optimization of zinc acetate (1.5 g), plant extract (5 mL), pH 12, and temperature (25 °C), and using the stability study (absorbance between 365-370 nm) and characterization data exhibiting broad and shifted bands (in FTIR spectrum), the size was found to be below 98.61 nm (determined by FESEM and XRD spectra) and 71.54% zinc was observed in the EDX spectrum. Biogenic ZnPs exhibited a high inhibitory activity against periimplantitis-triggering pathogens ( and ). Based on the experimental results, the present study concludes that biogenic ZnPs possess a high inhibitory potential against periimplantitis-triggering bacteria, and it is established that the biosynthesis of ZnPs using APLAE is a useful method.
由致病细菌引起的种植体周围炎被认为是牙种植体失败的主要原因。已知生物源氧化锌纳米颗粒（ZnPs）可抑制引发种植体周围炎的病原体。当前的研究旨在进行ZnPs的生物合成并评估其对引发种植体周围炎细菌的作用。当前的研究包括使用叶片水提取物（APLAE）进行ZnPs的生物合成，随后进行优化、稳定性研究、表征以及对引发种植体周围炎细菌的体外评估。实验结果表明，基于醋酸锌（1.5 g）、植物提取物（5 mL）、pH 12和温度（25°C）的优化，成功合成了ZnPs，并通过稳定性研究（吸光度在365 - 370 nm之间）以及表征数据（傅里叶变换红外光谱中的宽峰和位移峰）表明，通过场发射扫描电子显微镜和X射线衍射光谱测定其尺寸低于98.61 nm，能量散射X射线光谱显示锌含量为71.54%。生物源ZnPs对引发种植体周围炎的病原体表现出高抑制活性。基于实验结果，本研究得出结论，生物源ZnPs对引发种植体周围炎的细菌具有高抑制潜力，并且证实使用APLAE生物合成ZnPs是一种有用的方法。
