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从红厚壳合成的氧化锌纳米颗粒对HCC2998结肠癌细胞的氧化应激诱导细胞毒性作用。

Oxidative stress-induced cytotoxicity of HCC2998 colon carcinoma cells by ZnO nanoparticles synthesized from Calophyllum teysmannii.

作者信息

Subramaniam Hemaroopini, Lim Chan Kiang, Tey Lai Hock, Wong Ling Shing, Djearamane Sinouvassane

机构信息

Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, Kampar, Perak, 31900, Malaysia.

Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar Barat, Kampar, Perak, 31900, Malaysia.

出版信息

Sci Rep. 2024 Dec 4;14(1):30198. doi: 10.1038/s41598-024-81384-0.

DOI:10.1038/s41598-024-81384-0
PMID:39632962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11618351/
Abstract

The field of green synthesis, namely using plant extracts for the production of metal nanoparticles, is rapidly gaining traction. Therefore, this study investigated the process of producing zinc oxide nanoparticles (ZnO NPs) using a water-based extract derived from the stem bark of Calophyllum teysmannii. Notably, this is the first documented utilization of this particular plant source. The presence of a distinct Ultraviolet-Visible (UV-Vis) absorption peak at 372 nm provided evidence for the creation of ZnO nanoparticles. The X-ray Diffractometer (XRD) and Field Emission Scanning Electron Microscopy (FESEM) investigations indicated that the nanoparticles exhibited sizes ranging from 31.5 to 59.9 nm and had spherical morphologies. Energy Dispersive X-ray Diffractometer (EDX) analysis verified the elemental composition of the ZnO nanoparticles, whereas the Fourier Transform Infrared (FTIR) spectra showed clear peaks, demonstrating their production. The FTIR examination of the C. teysmannii extract revealed peaks at around 3370 cm, indicating the presence of phenolic compounds. These chemicals are likely responsible for the reduction and stabilization of the ZnO NPs. The high-resolution X-ray Photoelectron Spectroscopy (XPS) spectra clearly revealed separate peaks corresponding to Zn 2p and O 1s, providing confirmation of the chemical states and bonding contexts. The Raman Spectroscopy analysis revealed a distinct peak at around 425 cm⁻¹, confirming the presence of the wurtzite structure. The harmful effects of ZnO nanoparticles on HCC2998 (a kind of human colon cancer) and Vero (a type of monkey kidney epithelial) cells were evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT), dichlorodihydrofluorescein diacetate (DCFH-DA), and boron-Dipyrromethene (BODIPY) assays. The cancer cells underwent cell death due to oxidative stress in a dose-dependent manner, as confirmed by microscopic and flow cytometry investigations.

摘要

绿色合成领域，即利用植物提取物生产金属纳米颗粒，正在迅速获得关注。因此，本研究调查了使用从泰氏红厚壳茎皮中提取的水基提取物生产氧化锌纳米颗粒（ZnO NPs）的过程。值得注意的是，这是首次有文献记载使用这种特定植物来源。在372 nm处出现明显的紫外可见（UV-Vis）吸收峰，为ZnO纳米颗粒的生成提供了证据。X射线衍射仪（XRD）和场发射扫描电子显微镜（FESEM）研究表明，纳米颗粒的尺寸范围为31.5至59.9 nm，且具有球形形态。能量色散X射线衍射仪（EDX）分析验证了ZnO纳米颗粒的元素组成，而傅里叶变换红外（FTIR）光谱显示出明显的峰，证明了它们的生成。对泰氏红厚壳提取物的FTIR检测显示在约3370 cm处有峰，表明存在酚类化合物。这些化学物质可能负责ZnO NPs的还原和稳定。高分辨率X射线光电子能谱（XPS）光谱清楚地显示了对应于Zn 2p和O 1s的单独峰，证实了化学状态和键合情况。拉曼光谱分析在约425 cm⁻¹处显示出一个明显的峰，证实了纤锌矿结构的存在。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）、二氯二氢荧光素二乙酸酯（DCFH-DA）和硼-二吡咯亚甲基（BODIPY）测定法评估了ZnO纳米颗粒对HCC2998（一种人类结肠癌细胞）和Vero（一种猴肾上皮细胞）的有害影响。显微镜和流式细胞术研究证实，癌细胞因氧化应激以剂量依赖的方式发生细胞死亡。

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