Department of Hepatobiliary, Xi'an no. 3 Hospital, the Affiliated Hospital of Northwest University, 710018, Xi'an, Shaanxi, China.
Department of Medicinal Chemistry, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
Dokl Biochem Biophys. 2021 Sep;500(1):360-367. doi: 10.1134/S1607672921050148. Epub 2021 Oct 25.
Zinc oxide nanoparticles (ZnO NPs) have peaked interests in many researches in these recent years due to their advantageous application in modern health care applications. Therefore, we successfully synthesized ZnO NPs by Acacia luciana flower extract as stabilizing, reducing and capping agent, to investigate the antiproliferative potential and apoptosis induction in breast cancer cell lines. The involvements of Acacia luciana bioactive compounds in the stabilization of the ZnO NPs were confirmed by FTIR analysis. FESEM and EDS instruments confirmed that biosynthesized nanoparticles have an irregular morphology and mostly composed of Zn, C, and O respectively. The TEM and zeta potential instruments confirmed that biosynthesized nanoparticles have slight negative charges with particle size of 40 nm. The survivorship of MCF-7 cells were examined by MTT assay and revealed that ZnO NPs inhibited cell viability in a dose- and time-dependent effect with IC value of 3.1 µg/mL after 72 h exposure. Also, as a novel work onto ZnO NPs obtained by Acacia extracts, the Bak1/Bclx expression ratio was elucidated utilizing RT-PCR technique. The results demonstrated that ZnO NPs could enhance the expression ratio; therefore they have the potential to induce apoptosis in breast cancer cells via mitochondria-mediated apoptotic pathway.
氧化锌纳米粒子（ZnO NPs）由于在现代医疗保健应用中的优势而引起了许多研究的关注。因此，我们成功地使用金合欢花提取物作为稳定、还原和封端剂合成了 ZnO NPs，以研究其在乳腺癌细胞系中的抗增殖潜力和凋亡诱导作用。FTIR 分析证实了金合欢花生物活性化合物在 ZnO NPs 稳定中的参与。FESEM 和 EDS 仪器证实，生物合成的纳米粒子具有不规则的形态，主要由 Zn、C 和 O 组成。TEM 和 zeta 电位仪器证实，生物合成的纳米粒子带有轻微的负电荷，粒径为 40nm。MTT 测定法检测 MCF-7 细胞的存活率，结果表明 ZnO NPs 以剂量和时间依赖性方式抑制细胞活力，72 h 暴露后 IC 值为 3.1μg/mL。此外，作为金合欢提取物获得的 ZnO NPs 的一项新工作，利用 RT-PCR 技术阐明了 Bak1/Bclx 表达比值。结果表明，ZnO NPs 可以增强表达比值，因此它们有可能通过线粒体介导的凋亡途径诱导乳腺癌细胞凋亡。
