Uddin Nisar, Muhammad Noor, Nisar Mohammad, Ali Niaz, Ullah Riaz, Ali Essam A, Khan Azhar Abbas, Rahman Inayat Ur, Khan Anwar, Zeb Alam
Department of Botany Hazara University Mansehra Mansehra Pakistan.
Department of Pomology College of Horticulture Hebei Agricultural University Baoding China.
Food Sci Nutr. 2022 Jan 28;10(5):1414-1430. doi: 10.1002/fsn3.2726. eCollection 2022 May.
fruits have attracted much attention within the field of medicine due to their high potential against central nervous system disorders. Abundance of secondary metabolites and their composition is key to the pharmaceutical potential and commercial qualities of plants. The in vitro antioxidant activities of (Burm. f.) and Edgew fruit extract were analyzed using 2,2-diphenil-1-pycrilhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) free radical scavenging assay methods. Phenolic profiles were explored using high-performance liquid chromatography-diode array detector (HPLC-DAD). The result revealed high concentration of polyphenols and their antioxidant potential. In , the total phenolic content (TPC) (80.270 ± 0.422 μg/ml), DPPH (62.03 ± 0.98 μg/ml), ABTS (66.32 ± 0.73 μg/ml), and TFC (90.683 ± 0.274 μg/ml) were recorded. However, in , DPPH and ABTS values were 60.66 ± 0.56 μg/ml and 61.55 ± 0.77 μg/ml, respectively, indicative of the impacts of climate and soil nutrients. The overall screening of phytochemicals revealed that both the species contain diverse bioactive compounds, including spinacetine-3-O-(2 feruloyl glucopyranosyl)-glucopyranoside, kaempferol-3-O-glucoside-7-O-glucoside, and caffeic acid; p-hydroxybenzoyl hexose, p-coumaric acid, salicylic acid, and ellagic acid pentoxide. Additionally, the highest concentrated amino acid noted was of Lue 0.19 g/100 g with 596.00 retention time (RT), followed by Thr>Ale>Isl>Phya>Val in . Similarly, the highest concentration of Lue amino acid was recorded as 0.18/100 g with 564.52 RT followed by Pr>Thr>Ale>Lue>Isl>Phya>Val in all genotypes of . Reporting of polyphenols rich and stable species along with identification of favorable regions of cultivation for amino acid, polyphenols, and higher antioxidant potential may lead the way for the identification of elite clones of the species as well as may result in new drug discovery.
由于水果对中枢神经系统疾病具有很高的防治潜力，因此在医学领域备受关注。丰富的次生代谢产物及其组成是植物药用潜力和商业品质的关键。采用2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除测定法分析了（Burm. f.）和Edgew果实提取物的体外抗氧化活性。使用高效液相色谱-二极管阵列检测器（HPLC-DAD）探究酚类成分。结果显示多酚含量高及其具有抗氧化潜力。在中，记录到总酚含量（TPC）（80.270±0.422μg/ml）、DPPH（62.03±0.98μg/ml）、ABTS（66.32±0.73μg/ml）和总黄酮含量（TFC）（90.683±0.274μg/ml）。然而，在中，DPPH和ABTS值分别为60.66±0.56μg/ml和61.55±0.77μg/ml，表明气候和土壤养分的影响。植物化学物质的全面筛选表明，这两个物种都含有多种生物活性化合物，包括菠菜碱-3-O-（2-阿魏酰葡萄糖吡喃糖基）-葡萄糖吡喃糖苷、山奈酚-3-O-葡萄糖苷-7-O-葡萄糖苷和咖啡酸；对羟基苯甲酰己糖、对香豆酸、水杨酸和五氧化二鞣花酸。此外，记录到最高浓度氨基酸为亮氨酸0.19g/100g，保留时间（RT）为596.00，其次是苏氨酸>丙氨酸>异亮氨酸>苯丙氨酸>缬氨酸。同样，在的所有基因型中，亮氨酸的最高浓度记录为0.18/100g，RT为564.52，其次是脯氨酸>苏氨酸>丙氨酸>亮氨酸>异亮氨酸>苯丙氨酸>缬氨酸。报告富含多酚且稳定的物种，同时鉴定氨基酸、多酚和更高抗氧化潜力的有利种植区域，可能为鉴定该物种的优良克隆指明方向，也可能带来新药发现。
