MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Engineering, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Integrated Chinese & Western Medicine Oncology Research Center, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330004, China.
Analyst. 2022 Jun 13;147(12):2671-2677. doi: 10.1039/d2an00370h.
Photothermal biosensors with advantages of speed and high sensitivity offer alternative and reliable solutions for real-time clinical diagnosis, food safety testing and environmental monitoring. Although metallic nanoparticles are usually used for photothermal biosensors, their poor photothermal stability and potential toxicity hinder clinical applications. Taking advantage of the low cytotoxicity and remarkable photothermal effect under the low laser power of polypyrrole-based organic nanoparticles, we developed a novel photothermal biosensor with a temperature and pressure dual readout. After the formation of immunoassay sandwich structures, polypyrrole as the photothermal agent is synthesized with pyrrole, HCl and Fe released from magnetic FeO particles modified with detection antibody. The heterocyclic rings from polypyrrole enable photothermal performance in the NIR region. The resulting increased heat and pressure in a sealed well are measured using a digital thermometer and a portable pressure meter, respectively. Taking C-reactive protein (CRP) as a model target, the proposed strategy allowed sensitive, selective and accurate analysis of biomarkers, and showed performance comparable to that of ELISA. Overall, the dual-mode photothermal biosensor holds great potential for simple and low-cost photothermal sensing of biomarkers for point-of-care testing (POCT).
光热生物传感器具有速度快、灵敏度高的优点，为实时临床诊断、食品安全检测和环境监测提供了替代和可靠的解决方案。虽然金属纳米粒子通常用于光热生物传感器，但它们较差的光热稳定性和潜在的毒性阻碍了临床应用。利用基于聚吡咯的有机纳米粒子在低激光功率下的低细胞毒性和显著的光热效应，我们开发了一种具有温度和压力双读数的新型光热生物传感器。在形成免疫分析三明治结构后，用从修饰有检测抗体的磁性 FeO 粒子中释放出的吡咯、HCl 和 Fe 合成聚吡咯作为光热剂。聚吡咯中的杂环使其在近红外区域具有光热性能。通过数字温度计和便携式压力计分别测量密封井中产生的热量和压力的增加。以 C 反应蛋白 (CRP) 为模型靶标，该策略实现了对生物标志物的灵敏、选择性和准确分析，性能可与 ELISA 相媲美。总的来说，这种双模光热生物传感器在用于即时检测（POCT）的生物标志物的简单、低成本光热传感方面具有很大的潜力。
