Center for Musculoskeletal Regeneration, Houston Methodist Academic Institute, Houston, TX, USA; Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy.
Center for Musculoskeletal Regeneration, Houston Methodist Academic Institute, Houston, TX, USA; Department of Molecular Medicine, Texas A&M MD/PhD Program, Texas A&M Health Science Center, College Station, TX, USA.
Nanomedicine. 2022 Aug;44:102567. doi: 10.1016/j.nano.2022.102567. Epub 2022 May 18.
Myocardial infarction remains the leading cause of death in the western world. Since the heart has limited regenerative capabilities, several cardiac tissue engineering (CTE) strategies have been proposed to repair the damaged myocardium. A novel electrospun construct with aligned and electroconductive fibers combining gelatin, poly(lactic-co-glycolic) acid and polypyrrole that may serve as a cardiac patch is presented. Constructs were characterized for fiber alignment, surface wettability, shrinkage and swelling behavior, porosity, degradation rate, mechanical properties, and electrical properties. Cell-biomaterial interactions were studied using three different types of cells, Neonatal Rat Ventricular Myocytes (NRVM), human lung fibroblasts (MRC-5) and induced pluripotent stem cells (iPSCs). All cell types showed good viability and unique organization on construct surfaces depending on their phenotype. Finally, we assessed the maturation status of NRVMs after 14 days by confocal images and qRT-PCR. Overall evidence supports a proof-of-concept that this novel biomaterial construct could be a good candidate patch for CTE applications.
心肌梗死仍然是西方世界的主要死因。由于心脏的再生能力有限，已经提出了几种心脏组织工程 (CTE) 策略来修复受损的心肌。本文提出了一种具有取向和导电性纤维的新型静电纺丝构建体，该构建体由明胶、聚（乳酸-共-乙醇酸）和聚吡咯组成，可用作心脏补片。对构建体的纤维取向、表面润湿性、收缩和溶胀行为、孔隙率、降解率、力学性能和电学性能进行了表征。使用三种不同类型的细胞（乳鼠心室肌细胞 (NRVM)、人肺成纤维细胞 (MRC-5) 和诱导多能干细胞 (iPSC)）研究了细胞-生物材料相互作用。所有细胞类型在构建体表面均表现出良好的活力和独特的组织，这取决于它们的表型。最后，我们通过共聚焦图像和 qRT-PCR 评估了 NRVMs 在 14 天后的成熟状态。总体证据支持这样一种观点，即这种新型生物材料构建体可能是 CTE 应用的良好候选补片。
