Department of Genetics, Faculty of Natural and Agricultural Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
ThermoFisher Scientific, Fairland, Johannesburg 2195, South Africa.
Sci Justice. 2022 May;62(3):358-364. doi: 10.1016/j.scijus.2022.03.009. Epub 2022 Mar 31.
South Africa has one of the highest rape statistics in the world, with an average of 117 rapes reported daily. Y-STR genotyping is becoming a popular tool in the analysis of DNA evidence collected after a crime of a sexual nature has been committed, but has yet to be implemented in South Africa's forensic laboratories. This study aimed to investigate the forensic value of the 27 Yfiler™ Plus loci in the South African population. A total of 271 samples from the African, Asian/Indian, Mixed Ancestry, and Caucasian populations at the University of the Free State in Bloemfontein, South Africa were amplified and analysed using ThermoFisher Scientific's Yfiler™ Plus PCR Amplification kit. Of the 271 samples, 261 were identified to be unique, with an overall discrimination capacity of 98.15%. Discrimination capacities ranged from 91.67% for the Asian/Indian population to 100% for the Mixed Ancestry population. The haplotype diversity across the four populations is 0.9999, with an average gene diversity across all loci of 0.717. The forensic parameters estimated in this study provide evidence for the potential use of the commercial Yfiler™ Plus PCR amplification kit in a forensic application in South Africa.
南非的强奸犯罪率位居世界前列，平均每天报告 117 起强奸案。Y-STR 基因分型在分析性犯罪后收集的 DNA 证据方面正成为一种流行的工具，但尚未在南非法医实验室中实施。本研究旨在调查 27 个 Yfiler™ Plus 基因座在南非人群中的法医学价值。共扩增和分析了来自南非布隆方丹自由州大学的 271 个非洲人、亚洲/印度人、混血人群和高加索人群样本，使用的是赛默飞世尔科技的 Yfiler™ Plus PCR 扩增试剂盒。在 271 个样本中，有 261 个样本被鉴定为独特，总体鉴别能力为 98.15%。鉴别能力范围从亚洲/印度人群的 91.67%到混血人群的 100%。四个群体的单倍型多样性为 0.9999，所有基因座的平均基因多样性为 0.717。本研究中估计的法医学参数为在南非的法医应用中使用商业 Yfiler™ Plus PCR 扩增试剂盒提供了证据。
