Huang Yingzhao, Zhan Qi, Wu Chenzhou, Liao Nailin, Jiang Zhou, Ding Haoran, Wang Kunyu, Li Yi
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Front Pharmacol. 2022 May 4;13:868398. doi: 10.3389/fphar.2022.868398. eCollection 2022.
Nanoparticles for the gene therapy field have seen remarkable progress over the last 10 years; however, low delivery efficiency and other reasons impede the clinical translation of nanocarriers. Therefore, a summary of hotspots and trends in this field is needed to promote further research development. In this research, from 2011 to 2021, 1,221 full records and cited references of Web of Science-indexed manuscripts regarding nanoparticle-targeted delivery systems have been analyzed by CiteSpace, VOSviewer, and MapEquation. In these software, keywords co-occurrence networks, alluvial diagram, co-citation networks, and structural variation analysis were carried out to emphasize the scientific community's focus on nanomedicine of targeted delivering of nucleic acids. Keywords such as transfection efficiency, tumor cell, membrane antigen, and siRNA delivery were highlighted in the density map from VOSviewer. In addition, an alluvial flow diagram was constructed to detect changes in concepts. In the co-citation network, cluster 1 (exosomes) and cluster 17 (genome editing) were new research fields, and the efforts in modifying nanoparticles were revealed in the structural variation analysis. Aptamer and SELEX (systematic evolution of ligands by exponential enrichment) represented a helpful system in targeted delivery. These results indicated that the transfection efficiency of nanocarriers required continuous improvements. With the approval of several nucleic acid drugs, a new content of nanoparticle carriers is to introduce gene-editing technology, especially CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9). In addition, exosomes have great potential as targeted nanoparticles. By mapping the knowledge domains of nanomedicine in targeted delivering of nucleic acids, this study analyzed the intellectual structure of this domain in the recent 10 years, highlighting classical modifications on nanoparticles and estimating future trends for researchers and decision-makers interested in this field.
在过去十年中，用于基因治疗领域的纳米颗粒取得了显著进展；然而，低递送效率和其他因素阻碍了纳米载体的临床转化。因此，需要对该领域的热点和趋势进行总结，以促进进一步的研究发展。在本研究中，通过CiteSpace、VOSviewer和MapEquation对2011年至2021年Web of Science索引的关于纳米颗粒靶向递送系统的1221篇完整记录及引用文献进行了分析。在这些软件中，进行了关键词共现网络、冲积图、共被引网络和结构变异分析，以强调科学界对核酸靶向递送纳米医学的关注。VOSviewer的密度图突出显示了转染效率、肿瘤细胞、膜抗原和siRNA递送等关键词。此外，构建了冲积流程图以检测概念的变化。在共被引网络中，聚类1（外泌体）和聚类17（基因组编辑）是新的研究领域，结构变异分析揭示了在修饰纳米颗粒方面所做的努力。适体和SELEX（指数富集配体系统进化）代表了一种有用的靶向递送系统。这些结果表明，纳米载体的转染效率需要不断提高。随着几种核酸药物的获批，纳米颗粒载体的一个新内容是引入基因编辑技术，尤其是CRISPR/Cas9（成簇规律间隔短回文重复序列/CRISPR相关蛋白9）。此外，外泌体作为靶向纳米颗粒具有巨大潜力。通过绘制核酸靶向递送中纳米医学的知识领域图，本研究分析了该领域近十年的知识结构，突出了纳米颗粒的经典修饰，并为该领域感兴趣的研究人员和决策者预测了未来趋势。
