长链非编码RNA HCP5通过靶向微小RNA-128-3p促进鼻咽癌进展。
Long Noncoding RNA HCP5 Contributes to Nasopharyngeal Carcinoma Progression by Targeting MicroRNA-128-3p.
作者信息
Miao Gangyong, Liu Bin, Ling Keji, Peng Tao, Zhou En, Xie Sijun, Tan Zhiqiang
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Hunan Provincial People's Hospital, Changsha 410005, China.
Department of Ophthalmology, 2nd Affiliated Hospital of Xiangya College, Central South University, Changsha, China.
出版信息
J Oncol. 2022 May 12;2022:5740857. doi: 10.1155/2022/5740857. eCollection 2022.
AIM
To determine the role and underlying mechanism of lncRNA HCP5 in nasopharyngeal carcinoma (NPC).
METHOD
The expression of HCP5 and miR-128-3p was assessed by qRT-PCR. CCK-8, EdU staining, and transwell were performed to determine cell progression. A nude mouse xenograft tumor model was carried out to detect the role of HCP5 . The luciferase assay was performed to confirm the function between lncRNA HCP5 and miR-128-3p.
RESULTS
The increased level of HCP5 was observed in NPC tissues. Silencing of HCP5 prevented tumor progression and . The luciferase assay verified that HCP5 could bind with miR-128-3p. Furthermore, forced expression of miR-128-3p could prevent the function of HCP5 on NPC cells.
CONCLUSION
lncRNA HCP5 could regulate NPC cell progression sponging miR-128-3p, which might serve as a potential therapy target of NPC.
目的
确定长链非编码RNA HCP5在鼻咽癌（NPC）中的作用及潜在机制。
方法
采用qRT-PCR检测HCP5和miR-128-3p的表达。进行CCK-8、EdU染色和transwell实验以确定细胞进展。建立裸鼠异种移植瘤模型以检测HCP5的作用。进行荧光素酶报告基因检测以确认长链非编码RNA HCP5与miR-128-3p之间的功能关系。
结果
在NPC组织中观察到HCP5水平升高。沉默HCP5可阻止肿瘤进展。荧光素酶报告基因检测证实HCP5可与miR-128-3p结合。此外，强制表达miR-128-3p可阻止HCP5对NPC细胞的作用。
结论
长链非编码RNA HCP5可通过海绵吸附miR-128-3p调节NPC细胞进展，这可能成为NPC的潜在治疗靶点。
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