Characterising the proteomic response of mushroom pathogen to QST 713 and Kos biocontrol agents.

UNLABELLED

The fungal pathogen causes dry bubble disease in cultivation and affected mushrooms significantly reduce the yield and revenue for mushroom growers. Biocontrol agents may represent an alternative and more environmentally friendly treatment option to help control dry bubble on mushroom farms. Serenade ® is a commercially available biocontrol product used for disease treatment in plant crops. In this work, the in vitro response of to the bacterial strain active in Serenade, (QST 713) and a newly isolated strain (Kos) was assessed. (QST713 and Kos) both produced zones of inhibition on plate cultures of , reduced the mycelium growth in liquid cultures and damaged the morphology and structure of hyphae. The proteomic response of the pathogen against these biocontrol strains was also investigated. Proteins involved in growth and translation such as 60S ribosomal protein L21-A (-32-fold) and 40S ribosomal protein S30 (-17-fold) were reduced in abundance in QST 713 treated samples, while proteins involved in a stress response were increased (norsolorinic acid reductase B (47-fold), isocitrate lyase (11-fold) and isovaleryl-CoA dehydrogenase (8-fold). was found to have a similar proteomic response when exposed to (Kos). This work provides information on the response of to (QST 713) and indicates the potential of Kos as a novel biocontrol agent.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s10658-022-02482-1.