Clarke Joy, Grogan Helen, Fitzpatrick David, Kavanagh Kevin
Department of Biology, Maynooth University, Maynooth, Kildare Ireland.
Teagasc, Horticulture Development Department, Ashtown Research Centre, Dublin 15, Ireland.
Eur J Plant Pathol. 2022;163(2):369-379. doi: 10.1007/s10658-022-02482-1. Epub 2022 Apr 22.
The fungal pathogen causes dry bubble disease in cultivation and affected mushrooms significantly reduce the yield and revenue for mushroom growers. Biocontrol agents may represent an alternative and more environmentally friendly treatment option to help control dry bubble on mushroom farms. Serenade ® is a commercially available biocontrol product used for disease treatment in plant crops. In this work, the in vitro response of to the bacterial strain active in Serenade, (QST 713) and a newly isolated strain (Kos) was assessed. (QST713 and Kos) both produced zones of inhibition on plate cultures of , reduced the mycelium growth in liquid cultures and damaged the morphology and structure of hyphae. The proteomic response of the pathogen against these biocontrol strains was also investigated. Proteins involved in growth and translation such as 60S ribosomal protein L21-A (-32-fold) and 40S ribosomal protein S30 (-17-fold) were reduced in abundance in QST 713 treated samples, while proteins involved in a stress response were increased (norsolorinic acid reductase B (47-fold), isocitrate lyase (11-fold) and isovaleryl-CoA dehydrogenase (8-fold). was found to have a similar proteomic response when exposed to (Kos). This work provides information on the response of to (QST 713) and indicates the potential of Kos as a novel biocontrol agent.
The online version contains supplementary material available at 10.1007/s10658-022-02482-1.
真菌病原体在蘑菇栽培中引发干泡病，受影响的蘑菇会显著降低蘑菇种植者的产量和收益。生物防治剂可能是一种替代性的、更环保的处理选择，有助于控制蘑菇农场中的干泡病。小檗碱（Serenade®）是一种可用于植物作物疾病治疗的商业生物防治产品。在这项研究中，评估了蘑菇病原菌对小檗碱中活性细菌菌株枯草芽孢杆菌（Bacillus subtilis）QST 713和新分离的枯草芽孢杆菌菌株（Kos）的体外反应。枯草芽孢杆菌QST713和Kos在蘑菇病原菌的平板培养物上均产生了抑菌圈，降低了液体培养物中菌丝体的生长，并破坏了蘑菇病原菌菌丝的形态和结构。还研究了病原菌对这些生物防治菌株的蛋白质组反应。在经枯草芽孢杆菌QST 713处理的样品中，参与生长和翻译的蛋白质，如60S核糖体蛋白L21-A（-32倍）和40S核糖体蛋白S30（-17倍）的丰度降低，而参与应激反应的蛋白质增加（诺索洛林酸还原酶B（47倍）、异柠檬酸裂解酶（11倍）和异戊酰辅酶A脱氢酶（8倍））。发现当暴露于枯草芽孢杆菌（Kos）时，蘑菇病原菌有类似的蛋白质组反应。这项研究提供了关于蘑菇病原菌对枯草芽孢杆菌（QST 713）反应的信息，并表明枯草芽孢杆菌Kos作为一种新型生物防治剂的潜力。
在线版本包含可在10.1007/s10658-022-02482-1获取的补充材料。
