互花米草入侵对亚热带海洋红树林生态系统氮固定和磷溶解的影响。
Effects of Spartina alterniflora Invasion on Nitrogen Fixation and Phosphorus Solubilization in a Subtropical Marine Mangrove Ecosystem.
机构信息
National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Biorefinery, Guangxi Research Center for Biological Science and Technology, Guangxi Academy of Sciences, Nanning, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi Universitygrid.256609.e, Nanning, China.
出版信息
Microbiol Spectr. 2022 Jun 29;10(3):e0068221. doi: 10.1128/spectrum.00682-21. Epub 2022 May 23.
Nitrogen fixation (NF) and phosphorus solubilization (PS) play a key role in maintaining the stability of mangrove ecosystems. In China, the invasion of Spartina alterniflora has brought a serious threat to the mangrove ecosystem. However, systematic research on NF and PS in mangrove sediments has not been conducted, and limited studies have focused on the response of NF and PS to invasion, particularly at different sediment depths. In the present study, shotgun metagenomics and quantitative PCR were used to study the 0- to 100-cm sediment profile of the mangrove ecosystem in the Beibu Gulf of China. Results showed that the PS potential of mangrove sediments was primarily caused by enzymes encoded by , , , , and genes. changed environmental factors, such as total nitrogen, total phosphorus, and total organic carbon, and enhanced the potential of NF and PS in sediments. Moreover, most microorganisms involved in NF or PS (NFOPSMs) responded positively to the invasion of . Cd, available iron, and salinity were the key environmental factors that affected the distribution of NF and PS genes (NFPSGs) and NFOPSMs. A strong coupling effect was observed between NF and PS in the mangrove ecosystem. invasion enhanced the coupling of NF and PS and the interaction of microorganisms involved in NF and PS (NFAPSM), thereby promoting the turnover of NP and improving sediment quality. Finally, 108 metagenome-assembled genomes involved in NF or PS were reconstructed to further evaluate NFOPSMs. This study revealed the efficient nutrient cycling mechanism of mangroves. Positive coupling effects were observed in sediment quality, NF and PS processes, and NFOPSMs with the invasion of . This research contributed to the understanding of the effects of invasion on the subtropical mangrove ecosystem and provided theoretical guidance for mangrove protection, restoration, and soil management. Additionally, novel NFOPSMs provided a reference for the development of marine biological fertilizers.
氮固定（NF）和磷溶解（PS）在维持红树林生态系统的稳定性方面发挥着关键作用。在中国，互花米草的入侵给红树林生态系统带来了严重威胁。然而，系统研究红树林沉积物中的 NF 和 PS 尚未开展，且对 NF 和 PS 对入侵的响应研究十分有限，尤其是在不同的沉积物深度下。本研究采用高通量宏基因组学和定量 PCR 技术，对中国北部湾红树林生态系统的 0-100cm 沉积物剖面进行了研究。结果表明，红树林沉积物的 PS 潜力主要由编码 、 、 、 和 基因的酶所驱动。 改变了总氮、总磷和总有机碳等环境因素，增强了沉积物中 NF 和 PS 的潜力。此外，大多数参与 NF 或 PS 的微生物（NFOPSMs）对 的入侵呈正响应。Cd、有效铁和盐度是影响 NF 和 PS 基因（NFPSGs）和 NFOPSMs 分布的关键环境因素。在红树林生态系统中观察到 NF 和 PS 之间存在强烈的耦合效应。 入侵增强了 NF 和 PS 的耦合以及参与 NF 和 PS 的微生物（NFAPSM）之间的相互作用，从而促进了 NP 的周转，改善了沉积物质量。最后，重建了 108 个与 NF 或 PS 相关的宏基因组组装基因组，以进一步评估 NFOPSMs。 本研究揭示了红树林高效的养分循环机制。在 入侵的情况下，观察到了沉积物质量、NF 和 PS 过程以及 NFOPSMs 之间的正耦合效应。本研究有助于理解 入侵对亚热带红树林生态系统的影响，为红树林保护、恢复和土壤管理提供了理论指导。此外，新发现的 NFOPSMs 为海洋生物肥料的开发提供了参考。
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