Jo Byeongwook, Morimoto Yuya, Takeuchi Shoji
Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
Adv Healthc Mater. 2022 Dec;11(24):e2200593. doi: 10.1002/adhm.202200593. Epub 2022 Jun 3.
In recent years, microfluidic systems have been extensively utilized for biological analysis. The integration of pumps in microfluidic systems requires precise control of liquids and effort-intensive set-ups for multiplexed experiments. In this study, a 3D-printed centrifugal pump driven by magnetic force is presented for microfluidics and biological analysis. The permanent magnets implemented in the centrifugal pump synchronized the rotation of the driving and operating parts. Precise control of the flow rate and a wide range and variety of flow profiles are achieved by controlling the rotational speed of the motor in the driving part. The compact size and contactless driving part allow simple set-ups within commercially available culture dishes and tubes. It is demonstrated that the fabricated 3D-printed centrifugal pump can induce laminar flow in a microfluidic device, perfusion culture of in vitro tissues, and alignment of cells under shear stress. This device has a high potential for applications in microfluidic devices and perfusion culture of cells.
近年来，微流控系统已被广泛用于生物分析。在微流控系统中集成泵需要精确控制液体，并且进行多重实验的设置工作强度大。在本研究中，提出了一种用于微流控和生物分析的由磁力驱动的3D打印离心泵。离心泵中使用的永磁体使驱动部件和操作部件的旋转同步。通过控制驱动部件中电机的转速，可以实现对流速的精确控制以及广泛多样的流动剖面。紧凑的尺寸和非接触式驱动部件允许在市售的培养皿和试管内进行简单的设置。结果表明，制造的3D打印离心泵可在微流控装置中诱导层流、进行体外组织的灌注培养以及在剪切应力下使细胞排列。该装置在微流控装置和细胞灌注培养方面具有很高的应用潜力。
