College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005390.
A novel bifidobacteria (designated S053-2) was isolated from the gut of honeybee (). Strain S053-2 was characterized using a polyphasic taxonomic approach. The result of 16S rRNA gene sequence analysis indicated that strain S053-2 was phylogenetically related to the type strains of , , , , , , and , and had 95.5-99.7 % 16S rRNA gene sequence similarities. Based on the 16S rRNA gene sequence analysis, strain S053-2 was most closely related to the type strain of , having 99.7 % 16S rRNA gene sequence similarity. Strain S053-2 had relatively low (91.6-95.7 %) , , , , , , , , , and sequence similarities to the type strain of . Strain S053-2 had 94.5-95.3% , , , and sequence similarities to the type strain of . The phylogenomic tree indicated that strain S053-2 belonged to the group, and was most closely related to the type strains of , , and , and distantly related to type strains of other phylogenetically related species in the group. Strain S053-2 shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with W8102. Strain S053-2 shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with DSM 20089. Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and -fucosidase could differentiate strain S053-2 from DSM 20089. Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of -fucosidase could differentiate strain S053-2 from W8102. Based upon the data obtained in the present study, a novel species, sp. nov., is proposed, and the type strain is S053-2 (=JCM 34710=CCTCC AB 2021129).
一株双歧杆菌（命名为 S053-2）从蜜蜂肠道中分离得到。S053-2 株通过多相分类学方法进行了鉴定。16S rRNA 基因序列分析结果表明，S053-2 株与、、、、、、和的模式菌株亲缘关系最近，与的 16S rRNA 基因序列相似性为 95.5-99.7%。基于 16S rRNA 基因序列分析，S053-2 株与的模式菌株最为密切相关，相似性为 99.7%。S053-2 株与的模式菌株的种间平均核苷酸相似性（ANI）、亲缘关系较远，分别为 91.6-95.7%、、、、、、、、和。S053-2 株与的模式菌株的 ANI、亲缘关系较远，分别为 94.5-95.3%、、、。系统发育树表明，S053-2 株属于双歧杆菌属，与、、和的模式菌株关系最近，与双歧杆菌属中其他系统发育相关种的模式菌株关系较远。S053-2 株与 W8102 株的平均核苷酸相似性（ANI）、数字 DNA-DNA 杂交（dDDH）和平均氨基酸相似性（AAI）最高，分别为 93.8%、52.4%和 95.6%。S053-2 株与 DSM 20089 株的 ANI、dDDH 和 AAI 值分别为 91.1%、41.9%和 92.5%。L-阿拉伯糖、D-木糖、D-甘露糖、苦杏仁苷、纤维二糖、麦芽糖、密二糖、蔗糖、棉子糖、龙胆二糖和 L-岩藻糖的酸产生以及酯酶脂肪酶（C8）和-岩藻糖苷酶的活性可将 S053-2 株与 DSM 20089 株区分开。D-甘露糖、麦芽糖、蔗糖、蜜二糖和龙胆二糖的酸产生以及-岩藻糖苷酶的活性可将 S053-2 株与 W8102 株区分开。基于本研究获得的数据，提出了一个新的物种，即双歧杆菌 sp. nov.，其模式菌株为 S053-2（=JCM 34710=CCTCC AB 2021129）。
