sp. nov. and sp. nov., isolated from traditional Chinese pickle.

Two Gram-stain-positive bacterial strains, designated 213-9(3) and 30-1(2), were isolated from traditional Chinese pickle, and were characterized using a polyphasic taxonomic approach. Results of 16S rRNA gene sequence analysis indicated that strain 213-9(3) was most closely related to TMW 1.1424, 151-2B and 220-4, having 99.7-99.9 % 16S rRNA gene sequence similarities; strain 30-1(2) was most closely related to 247-4, with 99.4 % 16S rRNA gene sequence similarity. Strain 213-9(3) shared the highest (93.9 %), (99.3 %) and concatenated and (97.5 %) sequence similarities to TMW 1.1424. Strain 30-1(2) had the highest (82.4 %), (95.5 %) and concatenated and (91.2 %) sequence similarities to 247-4. The phylogenetic relationships based on concatenated and sequences and whole genome sequences were identical to those based on 16S rRNA gene sequences. Strain 213-9(3) exhibited the highest average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values (92.7 and 48.8 %, respectively) to DSM 22467. Strain 30-1(2) had the highest ANI (84.4 %) and dDDH (32.8 %) values with 247-4. Acid production from d-galactose, d-glucose, d-mannose, -acetyl--glucosaminidase, arbutin, salicin, cellobiose, maltose, gentiobiose, d-tagatose and gluconate, hydrolysis of aesculin, and activity of cystine arylamidase could differentiate strain 213-9(3) from DSM 22467. Acid production from l-arabinose, d-ribose, d-xylose and d-galactose, and activity of alkaline phosphatase, esterase (C4), -mannosidase and -fucosidase could differentiate strain 30-1(2) from 247-4. Based upon the data obtained in the present study, two novel species, sp. nov. and sp. nov., are proposed and the type strains are 213-9(3) (=CCM 9241=CCTCC AB 2022115=JCM 35554) and 30-1(2) (=CCM 9240=CCTCC AB 2022114=JCM 35553), respectively.