新种及新种，从蜜蜂（）的肠道中分离得到。
sp. nov. and sp. nov., isolated from the gut of honeybee ().
机构信息
College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
出版信息
Int J Syst Evol Microbiol. 2024 Feb;74(2). doi: 10.1099/ijsem.0.006285.
Four lactic acid bacteria, designated F690, F697, F790 and F769-2, were isolated from the gut of honeybee (). Results of 16S rRNA gene sequence analysis indicated that strains F690 and F697 were phylogenetically related to the type strains of , , and , having 99.1-99.6 % 16S rRNA gene sequence similarities; and that strains F790 and F769-2 were most closely related to the type strain of , having 99.2-99.3 % 16S rRNA gene sequence similarities. The phylogenies based on concatenated , , , , , and sequences and based on whole genome sequences were identical to that based on 16S rRNA gene sequences. Strains F690 and F697 exhibited the highest average nucleotide identity (ANI; 92.1-93.2 %), digital DNA-DNA hybridization (dDDH; 50-50.1 %) and average amino acid identity (AAI; 94.9-95.1 %) values with Hma2N. Strains F790 and F769-2 had the highest ANI (93.1-94 %), dDDH (54.4 %) and AAI (94.4-94.7 %) values with Hma8N. Based upon the data obtained in the present study, two novel species, sp. nov. and sp. nov., are proposed and the type strains are F690 (=JCM 36259=CCTCC AB 2023131) and F790 (=JCM 36260=CCTCC AB 2023132), respectively.
从蜜蜂肠道中分离到 4 株乳酸菌，分别命名为 F690、F697、F790 和 F769-2。16S rRNA 基因序列分析结果表明，菌株 F690 和 F697 与 、 、 和 的模式菌株亲缘关系最近，16S rRNA 基因序列相似性为 99.1%-99.6%；菌株 F790 和 F769-2 与 的模式菌株亲缘关系最近，16S rRNA 基因序列相似性为 99.2%-99.3%。基于 、 、 、 、 和 序列的系统发育树以及全基因组序列的系统发育树与基于 16S rRNA 基因序列的系统发育树一致。菌株 F690 和 F697 与 Hma2N 的平均核苷酸同一性（ANI；92.1%-93.2%）、数字 DNA-DNA 杂交（dDDH；50%-50.1%）和平均氨基酸同一性（AAI；94.9%-95.1%）最高。菌株 F790 和 F769-2 与 Hma8N 的 ANI（93.1%-94%）、dDDH（54.4%）和 AAI（94.4%-94.7%）最高。根据本研究获得的数据，提出了两个新种，即 sp. nov.和 sp. nov.，其模式菌株分别为 F690（=JCM 36259=CCTCC AB 2023131）和 F790（=JCM 36260=CCTCC AB 2023132）。
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