Chaim-Matyas A, Ovadia M
Life Sci. 1987 Apr 20;40(16):1601-7. doi: 10.1016/0024-3205(87)90126-3.
Elapid, crotalid and viperid venoms were screened in vitro and in vivo for cytotoxicity towards B16F10 melanoma and chondrosarcoma cell lines. The cytotoxic activity of elapid venoms was considerably higher than that of viperid or crotalid venoms. Elapid venoms disrupted the cell membrane within the first hour, leading to cell death. The strongest activity was found in the venom of Naja nigricollis. The venoms of some Viperidae and of all Crotalidae examined caused the cells to become rounded, without loss in their original volume, and to form aggregates. These changes were reversible when cells were changed to fresh medium. In vivo experiments with the venom of Naja nigricollis were in total agreement with the results achieved in vitro with melanoma cells and the venom exhibited similar cytotoxic activity on chondrosarcoma, inhibiting its development in vivo.
对眼镜蛇科、蝰蛇科和蝮蛇科蛇毒进行了体外和体内实验，检测其对B16F10黑色素瘤细胞系和软骨肉瘤细胞系的细胞毒性。眼镜蛇科蛇毒的细胞毒性活性明显高于蝰蛇科或蝮蛇科蛇毒。眼镜蛇科蛇毒在最初一小时内破坏细胞膜，导致细胞死亡。在黑颈眼镜蛇的毒液中发现了最强的活性。所检测的一些蝰蛇科蛇毒和所有蝮蛇科蛇毒会使细胞变圆，细胞原始体积不变，并形成聚集体。当将细胞更换为新鲜培养基时，这些变化是可逆的。黑颈眼镜蛇毒液的体内实验结果与黑色素瘤细胞的体外实验结果完全一致，并且该毒液对软骨肉瘤表现出类似的细胞毒性活性，在体内抑制其生长。
