基于 MIP 仿生的高灵敏度阻抗传感器用于检测恩诺沙星。
A highly sensitive impedimetric sensor based on a MIP biomimetic for the detection of enrofloxacin.
机构信息
School of Engineering Physics, Hanoi University of Science and Technology, No. 1 Dai Co Viet road, Hai Ba Trung dist., Hanoi, Vietnam.
Vietnam-Korea Institute of Science and Technology, 304, 113 Tran Duy Hung, Cau Giay dist., Hanoi, Vietnam.
出版信息
Anal Methods. 2022 Jun 9;14(22):2195-2203. doi: 10.1039/d2ay00192f.
The benefits of molecularly imprinted polymer (MIP) technology in creating artificial receptors to replace natural counterparts have piqued the interest of numerous researchers in recent years. We propose a biomimetic enrofloxacin-MIP for enrofloxacin (ENRO) antigen detection using gold nanoparticles (AuNPs) and MIP methodologies in this study. A self-assembled monomer layer of aminothiophenol was used to immobilize a pre-formed complex of the anti-enrofloxacin monoclonal antibody and enrofloxacin antigen onto the surface of an AuNP coated screen-printed carbon ink electrode (SPCE). The poly-(aminothiophenol) layer thickness was adjusted to entrap and restrict enrofloxacin antigens near the surface. The imprinting and removal of the enrofloxacin antigen in the MIP film were strongly validated by the Raman spectra. The final mAb-MIP sensor had better sensitivity (302 Ω mL ng) and a better detection limit (0.05 ng mL) than self-assembled monolayer (SAM)-based immunosensors, which had 102 Ω mL ng and 0.1 ng mL, respectively.
近年来，分子印迹聚合物（MIP）技术在创造人工受体以替代天然受体方面的优势引起了众多研究人员的兴趣。本研究提出了一种仿生恩诺沙星-MIP，用于使用金纳米粒子（AuNPs）和 MIP 方法检测恩诺沙星（ENRO）抗原。采用氨巯基苯自组装单体层将抗恩诺沙星单克隆抗体和恩诺沙星抗原的预形成复合物固定在涂有金纳米粒子的丝网印刷碳油墨电极（SPCE）表面上。聚（氨巯基苯）层厚度被调整为将恩诺沙星抗原捕获并限制在表面附近。通过拉曼光谱对 MIP 膜中的恩诺沙星抗原的印迹和去除进行了强烈验证。最终的 mAb-MIP 传感器的灵敏度（302 Ω mL ng）和检测限（0.05 ng mL）优于基于自组装单分子层（SAM）的免疫传感器，后者的灵敏度分别为 102 Ω mL ng 和 0.1 ng mL。
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