Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021, China.
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, China.
Nanoscale. 2022 Jun 9;14(22):8112-8129. doi: 10.1039/d2nr02176e.
The repair of infected bone defects with irregular shapes is still a challenge in clinical work. Infected bone defects are faced with several major concerns: the complex shapes of bone defects, intractable bacterial infection and insufficient osseointegration. To solve these problems, we developed a personalized MXene composite hydrogel scaffold GelMA/β-TCP/sodium alginate (Sr)/MXene (TiC) (GTAM) with photothermal antibacterial and osteogenic abilities by 3D printing. , GTAM scaffolds could kill both Gram-positive and Gram-negative bacteria by NIR irradiation due to the excellent photothermal effects of MXene. Furthermore, rat bone marrow mesenchymal stem cells were mixed into GTAM bioinks for 3D bioprinting. The cell-laden 3D printed GTAM scaffolds showed biocompatibility and bone formation ability depending on MXene, crosslinked Sr, and β-TCP. , we implanted 3D printed GTAM scaffolds in -infected mandible defects of rats with NIR irradiation. GTAM scaffolds could accelerate the healing of infection and bone regeneration, and play synergistic roles in antibacterial and osteogenic effects. This study not only provides a strategy for the precise osteogenesis of infected bone defects, but also broadens the biomedical applications of MXene photothermal materials.
用不规则形状修复感染性骨缺损仍然是临床工作中的一个挑战。感染性骨缺损面临着几个主要问题：骨缺损的形状复杂、细菌感染难以控制和骨整合不足。为了解决这些问题，我们通过 3D 打印开发了一种具有光热抗菌和成骨能力的个性化 MXene 复合水凝胶支架 GelMA/β-TCP/海藻酸钠（Sr）/MXene（TiC）（GTAM）。由于 MXene 的优异光热效应，GTAM 支架可以通过 NIR 照射杀死革兰氏阳性菌和革兰氏阴性菌。此外，将大鼠骨髓间充质干细胞混入 GTAM 生物墨水进行 3D 生物打印。基于 MXene、交联 Sr 和 β-TCP，负载细胞的 3D 打印 GTAM 支架表现出良好的生物相容性和成骨能力。然后，我们在大鼠感染性下颌骨缺损中植入了经过 NIR 照射的 3D 打印 GTAM 支架。GTAM 支架可以加速感染的愈合和骨再生，并在抗菌和成骨效果方面发挥协同作用。本研究不仅为感染性骨缺损的精确成骨提供了策略，还拓宽了 MXene 光热材料的生物医学应用。
