Woo Sunghwa, Lim Hyun Gyu, Han Yong Hee, Park Sungwoo, Noh Myung Hyun, Baek Dongyeop, Moon Jo Hyun, Seo Sang Woo, Jung Gyoo Yeol
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, 37673, Gyeongbuk, Korea.
Interdisciplinary Program in Bioengineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Korea.
Biotechnol Biofuels Bioprod. 2022 May 25;15(1):58. doi: 10.1186/s13068-022-02157-3.
Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapidly and simultaneously consume three major lignocellulosic sugars (i.e., glucose, xylose, and arabinose) faster than any previously reported microorganisms.
The xylose isomerase pathway was constructed in Vibrio sp. dhg, which naturally displays high metabolic activities on glucose and arabinose but lacks xylose catabolism. Subsequent adaptive laboratory evolution significantly improved xylose catabolism of initial strain and led to unprecedently high growth and sugar uptake rate (0.67 h and 2.15 g g h, respectively). Furthermore, we achieved co-consumption of the three sugars by deletion of PtsG and introduction of GalP. We validated its superior performance and applicability by demonstrating efficient lactate production with high productivity (1.15 g/L/h) and titer (83 g/L).
In this study, we developed a Vibrio-based microbial platform with rapid and simultaneous utilization of the three major sugars from lignocellulosic biomass by applying an integrated approach of rational and evolutionary engineering. We believe that the developed strain can be broadly utilized to accelerate the production of diverse biochemicals from lignocellulosic biomass.
由于对气候变化和化石燃料枯竭的担忧日益增加，开发从木质纤维素生物质中高效生产生物化学物质的微生物工艺一直是一个关键问题。由于工艺效率受到宿主微生物固有代谢活性的极大影响，因此利用能够快速转化生物质衍生糖的微生物至关重要。在此，我们报告了一种基于弧菌的新型微生物平台，该平台能够比任何先前报道的微生物更快地同时快速消耗三种主要的木质纤维素糖（即葡萄糖、木糖和阿拉伯糖）。
在弧菌属dhg中构建了木糖异构酶途径，该菌株在葡萄糖和阿拉伯糖上天然具有高代谢活性，但缺乏木糖分解代谢能力。随后的适应性实验室进化显著改善了初始菌株的木糖分解代谢能力，并导致前所未有的高生长速率和糖摄取速率（分别为0.67 h和2.15 g g h）。此外，通过缺失PtsG和引入GalP，我们实现了三种糖的共同消耗。我们通过展示高效的乳酸生产，验证了其卓越的性能和适用性，乳酸生产具有高生产率（1.15 g/L/h）和产量（83 g/L）。
在本研究中，我们通过应用合理设计和进化工程的综合方法，开发了一种基于弧菌的微生物平台，该平台能够快速同时利用木质纤维素生物质中的三种主要糖。我们相信，所开发的菌株可广泛用于加速从木质纤维素生物质中生产各种生物化学物质。
