Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering , University of Freiburg , Georges-Koehler-Allee 103 , Freiburg 79110 , Germany.
ACS Appl Mater Interfaces. 2018 Nov 21;10(46):39411-39416. doi: 10.1021/acsami.8b11757. Epub 2018 Nov 9.
Biofunctional hydrogel particles have become increasingly popular in medical diagnostics; however, their generation is time-consuming and typically requires several process steps. We report on a new method for the simple, fast, and reproducible one-step generation of monodisperse hydrogel particles equipped with biofunctional molecules such as proteins or DNA. Key to the approach is the simultaneous photo cross-linking of the polymer chains and covalent binding of proteins or DNA through a C,H insertion reaction inside aqueous plug compartments that are produced via microfluidics. The strong performance in biological binding assays of the functionalized particles is demonstrated.
生物功能水凝胶颗粒在医学诊断中越来越受欢迎；然而，它们的生成耗时且通常需要多个处理步骤。我们报告了一种新方法，可简单、快速且可重复地一步生成带有生物功能分子（如蛋白质或 DNA）的单分散水凝胶颗粒。该方法的关键是通过微流控技术在水相微室中同时进行聚合物链的光交联和通过 C,H 插入反应将蛋白质或 DNA 共价结合。功能化颗粒在生物结合测定中的优异性能得到了证明。
