Wang Guisheng, Yin Zhenhua, Wang Senye, Yuan Yilin, Chen Yan, Kang Wenyi
National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China.
Joint International Research Laboratory of Food and Medicine Resource Function, Kaifeng, China.
Front Microbiol. 2022 May 10;13:900227. doi: 10.3389/fmicb.2022.900227. eCollection 2022.
In total, five new polyketide derivatives: eschscholin B (), dalditone A and B ( and ), (1, 4)-5-methoxy-1,2,3,4-tetrahydronaphthalene-1,4-dio (), and daldilene A (), together with 10 known as analogs (, -) were isolated from the mangrove endophytic fungus KBJYZ-1. Their structures and absolute configurations were established by extensive analysis of NMR and HRESIMS spectra data combined with ECD calculations and the reported literature. Compounds and showed significant cell-based anti-inflammatory activities with IC values of 19.3 and 12.9 μM, respectively. In addition, western blot results suggested that compound effectively inhibits the expression of iNOS and COX-2 in LPS-induced RAW264.7 cells. Further molecular biology work revealed the potential mechanism of exerts anti-inflammatory function by inactivating the MAPK and NF-κB signaling pathways.
从红树林内生真菌KBJYZ - 1中总共分离出5种新的聚酮化合物衍生物：eschscholin B（）、dalditone A和B（和）、（1, 4）-5 - 甲氧基 - 1,2,3,4 - 四氢萘 - 1,4 - 二酮（）以及daldilene A（），还有10种已知类似物（， - ）。通过对核磁共振（NMR）和高分辨电喷雾电离质谱（HRESIMS）光谱数据进行广泛分析，并结合电子圆二色（ECD）计算以及已报道的文献，确定了它们的结构和绝对构型。化合物和表现出显著的基于细胞的抗炎活性，其IC值分别为19.3和12.9 μM。此外，蛋白质印迹结果表明，化合物能有效抑制脂多糖（LPS）诱导的RAW264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶 - 2（COX - 2）的表达。进一步的分子生物学研究揭示了通过使丝裂原活化蛋白激酶（MAPK）和核因子κB（NF - κB）信号通路失活发挥抗炎功能的潜在机制。
