Buonaurio Flavia, Borra Francesca, Pigini Daniela, Paci Enrico, Spagnoli Mariangela, Astolfi Maria Luisa, Giampaoli Ottavia, Sciubba Fabio, Miccheli Alfredo, Canepari Silvia, Ancona Carla, Tranfo Giovanna
Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy.
Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, INAIL, Monte Porzio Catone, 00144 Rome, Italy.
Toxics. 2022 May 21;10(5):267. doi: 10.3390/toxics10050267.
The objective of this study is to evaluate the effects of traffic on human health comparing biomonitoring data measured during the COVID-19 lockdown, when restrictions led to a 40% reduction in airborne benzene in Rome and a 36% reduction in road traffic, to the same parameters measured in 2021.
Biomonitoring was performed on 49 volunteers, determining the urinary metabolites of the most abundant traffic pollutants, such as benzene and PAHs, and oxidative stress biomarkers by HPLC/MS-MS, 28 elements by ICP/MS and metabolic phenotypes by NMR.
Means of s-phenylmercaputric acid (SPMA), metabolites of naphthalene and nitropyrene in 2020 are 20% lower than in 2021, while 1-OH-pyrene was 30% lower. A reduction of 40% for 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodGuo) and 60% for 8-oxo-7,8-dihydroguanine (8-oxoGua) were found in 2020 compared to 2021. The concentrations of B, Co, Cu and Sb in 2021 are significantly higher than in the 2020. NMR untargeted metabolomic analysis identified 35 urinary metabolites. Results show in 2021 a decrease in succinic acid, a product of the Krebs cycle promoting inflammation.
Urban pollution due to traffic is partly responsible for oxidative stress of nucleic acids, but other factors also have a role, enhancing the importance of communication about a healthy lifestyle in the prevention of cancer diseases.
本研究的目的是通过比较在新冠疫情封锁期间测量的生物监测数据与2021年测量的相同参数，来评估交通对人类健康的影响。在封锁期间，限制措施使罗马空气中的苯含量降低了40%，道路交通量减少了36%。
对49名志愿者进行了生物监测，通过高效液相色谱-串联质谱法（HPLC/MS-MS）测定了最常见的交通污染物（如苯和多环芳烃）的尿代谢物以及氧化应激生物标志物，通过电感耦合等离子体质谱法（ICP/MS）测定了28种元素，并通过核磁共振（NMR）测定了代谢表型。
2020年s-苯基巯基尿酸（SPMA）、萘和硝基芘的代谢物平均值比2021年低20%，而1-羟基芘低30%。与2021年相比，2020年8-氧代-7,8-二氢鸟苷（8-oxoGuo）和8-氧代-7,8-二氢-2'-脱氧鸟苷（8-oxodGuo）降低了40%，8-氧代-7,8-二氢鸟嘌呤（8-oxoGua）降低了60%。2021年硼、钴、铜和锑的浓度显著高于2020年。核磁共振非靶向代谢组学分析确定了35种尿代谢物。结果显示，2021年促进炎症的三羧酸循环产物琥珀酸减少。
交通造成的城市污染部分导致了核酸的氧化应激，但其他因素也起了作用，这凸显了宣传健康生活方式在预防癌症疾病方面的重要性。
