Saha Sajjita, Unruh Jay R, Jaspersen Sue L
Stowers Institute for Medical Research.
Department of Molecular & Integrative Physiology, University of Kansas Medical Center.
MicroPubl Biol. 2021 Sep 16;2021. doi: 10.17912/micropub.biology.000464. eCollection 2021.
Microtubule nucleation is mediated by the conserved γ-tubulin ring complex (γ-TuRC). Using super-resolution microscopy, we investigate the distribution of γ-TuRC components at the spindle pole body (SPB) in wild-type . We observed asymmetric distribution of γ-TuRC on its nuclear and cytoplasmic surfaces, consistent with the uneven distribution of microtubules. Examination of deletion mutants in the three non-essential γ-TuRC subunits showed defects in γ-TuRC accumulation on the old and new SPB, particularly in cells lacking (the Gcp6 ortholog) that may explain the monopolar spindles observed in this mutant upon mitotic entry.
微管成核由保守的γ-微管蛋白环复合物（γ-TuRC）介导。利用超分辨率显微镜，我们研究了野生型中γ-TuRC组分在纺锤体极体（SPB）上的分布。我们观察到γ-TuRC在其核表面和胞质表面呈不对称分布，这与微管的不均匀分布一致。对三个非必需γ-TuRC亚基缺失突变体的检测显示，γ-TuRC在旧的和新的SPB上积累存在缺陷，特别是在缺乏（Gcp6直系同源物）的细胞中，这可能解释了该突变体在有丝分裂进入时观察到的单极纺锤体现象。
