Lin Yu-Hsuan, Yang Yi-Fang, Shiue Yow-Ling
Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Department of Otolaryngology, Head and Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
Diagnostics (Basel). 2022 May 9;12(5):1178. doi: 10.3390/diagnostics12051178.
() Previous studies have pointed out the significance of IgG Fc binding protein (FCGBP) in carcinogenesis, cancer progression, and tumor immunity in certain malignancies. However, its prognostic values, molecular interaction, and immune characteristics in the head and neck squamous cell carcinoma (HNSC) remained unclear. () To evaluate the potential role of the gene, we used GEPIA2 and UALCAN platforms to explore the differential levels, survivals, and genetic alteration through cBioPortal (based on The Cancer Genome Atlas dataset). STRING, GeneMania, and TIMER2.0 identified the interacting networks. LinkedOmics performed Gene enrichment analysis, and TISIDB and TIMER2.0 evaluated the role of in the tumor microenvironment. () The expression level of is lower in cancer tissues. A high level is significantly associated with better overall- and disease-specific-survivals, regardless of human papillomavirus infection. Low levels correlated to a higher ( mutation rate ( = 0.018). alteration significantly co-occurred with that of ( 0.037). Interacting networks revealed a significant association between FGFBP and trefoil factor 3 (TFF3), a novel prognostic marker in various cancers, at transcriptional and translational levels. Enrichment analyses identified that the top gene sets predominantly related to immune and inflammatory responses. Further investigation found that the mRNA level positively correlated to the infiltration rates of B cells, Th17/CD8+ T lymphocytes, T helper follicular cells, mast cells, and expression levels of various immune molecules and immune checkpoints in HNSC. () We found that the mRNA level negatively correlated to mutation status while positively correlated to the level. Additionally, FCGBP may regulate the tumor microenvironment. These findings support the as a potential biomarker to estimate HNSC prognoses.
（）先前的研究已经指出免疫球蛋白G Fc结合蛋白（FCGBP）在某些恶性肿瘤的致癌作用、癌症进展和肿瘤免疫中的重要性。然而，其在头颈部鳞状细胞癌（HNSC）中的预后价值、分子相互作用和免疫特征仍不清楚。（）为了评估该基因的潜在作用，我们使用GEPIA2和UALCAN平台，通过cBioPortal（基于癌症基因组图谱数据集）探索差异水平、生存率和基因改变。STRING、GeneMania和TIMER2.0确定了相互作用网络。LinkedOmics进行基因富集分析，TISIDB和TIMER2.0评估其在肿瘤微环境中的作用。（）该基因在癌组织中的表达水平较低。无论人乳头瘤病毒感染情况如何，高水平与更好的总生存率和疾病特异性生存率显著相关。低水平与较高的（突变率相关（=0.018）。改变与（的改变显著共发生（0.037）。相互作用网络显示FGFBP与三叶因子3（TFF3）之间存在显著关联，TFF3是各种癌症中的一种新型预后标志物，在转录和翻译水平上均如此。富集分析确定顶级基因集主要与免疫和炎症反应相关。进一步研究发现，该基因的mRNA水平与HNSC中B细胞、Th17/CD8 + T淋巴细胞、滤泡辅助性T细胞、肥大细胞的浸润率以及各种免疫分子和免疫检查点的表达水平呈正相关。（）我们发现该基因的mRNA水平与（突变状态呈负相关，而与（水平呈正相关。此外，FCGBP可能调节肿瘤微环境。这些发现支持该基因作为评估HNSC预后的潜在生物标志物。
