Song Chang-Ge, Chen Yuan-Yuan, Liu Shun, Xu Tai-Min, He Xiao-Lan, Wang Di, Cui Bao-Kai
Institute of Microbiology, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China.
College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.
J Fungi (Basel). 2022 Apr 22;8(5):429. doi: 10.3390/jof8050429.
In this study, phylogenetic analyses of from China were carried out based on sequences from the internal transcribed spacer (ITS) regions, the large subunit of nuclear ribosomal RNA gene (nLSU), the small subunit of nuclear ribosomal RNA gene (nSSU), the largest subunit of RNA polymerase II (RPB1), and the second largest subunit of RNA polymerase II (RPB2), combined with morphological characters of the collected specimens in China. The fruiting bodies of the specimens were used to observe their characteristics, and three new species of are discovered. is characterized by its pale brown to dark brown pileal surface, tomentose pileal margin, white spines, and the presence of clamp connections in generative hyphae of pileal surface, context, and stipe. is characterized by its dark brown to black pileal surface, white to pale brown pileal margin, the presence of both simple septa and clamp connections in generative hyphae of spines, and moderately long basidia. is characterized by its woody and broad pileus, brown to greyish brown pileal surface when fresh, tomentose pileal margin when young, which becomes glabrous with age, and the presence of both simple septa and clamp connections in the generative hyphae of the spines. This is the first time both single and multi-genes analysis is used in such a phylogenetic and taxonomic study on , which can provide the basis for the phylogenetic study of the genus.
在本研究中，基于来自中国的内部转录间隔区（ITS）序列、核糖体RNA基因大亚基（nLSU）、核糖体RNA基因小亚基（nSSU）、RNA聚合酶II最大亚基（RPB1）和RNA聚合酶II第二大亚基（RPB2），结合在中国采集的标本的形态特征，对来自中国的[具体物种名称未给出]进行了系统发育分析。利用标本的子实体观察其特征，发现了该[具体物种名称未给出]的三个新物种。[第一个新物种名称未给出]的特征在于其菌盖表面呈浅棕色至深棕色，菌盖边缘有绒毛，菌刺白色，且菌盖表面、菌肉和菌柄的生殖菌丝中有锁状联合。[第二个新物种名称未给出]的特征在于其菌盖表面呈深棕色至黑色，菌盖边缘白色至浅棕色，菌刺的生殖菌丝中有简单隔膜和锁状联合，且担子适度长。[第三个新物种名称未给出]的特征在于其菌盖木质且宽阔，新鲜时菌盖表面呈棕色至灰棕色，幼时菌盖边缘有绒毛，随着年龄增长变为无毛，且菌刺的生殖菌丝中有简单隔膜和锁状联合。这是首次在对[具体物种名称未给出]的此类系统发育和分类学研究中使用单基因和多基因分析，可为该属的系统发育研究提供依据。
