Abd-Rabboh Hisham S M, M Hashem Heba, M S Al Shagri Layla, E Amr Abdel El-Galil, Almehizia Abdulrahman A, Naglah Ahmed M, H Kamel Ayman
Chemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.
Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Membranes (Basel). 2022 Apr 21;12(5):446. doi: 10.3390/membranes12050446.
Novel cost-effective screen-printed potentiometric platforms for simple, fast, and accurate assessment of Fluoxetine (FLX) were designed and characterized. The potentiometric platforms integrate both the FLX sensor and the reference Ag/AgCl electrode. The sensors were based on the use of 4'-nitrobenzo-15-crown-5 (ionophore I), dibenzo-18-crown-6 (ionophore II), and 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) (ionophore III) as neutral carriers within a plasticized PVC matrix. Multiwalled carbon nanotubes (MWCNTs) were used as a lipophilic ion-to-electron transducing material and sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (NaTFPB) was used as an anionic excluder. The presented platforms revealed near-Nernstian potentiometric response with slopes of 56.2 ± 0.8, 56.3 ± 1.7 and 64.4 ± 0.2 mV/decade and detection limits of 5.2 × 10, 4.7 × 10 and 2.0 × 10 M in 10 mM Tris buffer solution, pH 7 for sensors based on ionophore I, II, and III, respectively. All measurements were carried out in 10 mM tris buffer solution at pH 7.0. The interfacial capacitance before and after insertion of the MWCNTs layer was evaluated for the presented sensors using the reverse-current chronopotentiometry. The sensors were introduced for successful determination of FLX drug in different pharmaceutical dosage forms. The results were compared with those obtained by the standard HPLC method. Recovery values were calculated after spiking fixed concentrations of FLX in different serum samples. The presented platforms can be potentially manufacturable at large scales and provide a portable, rapid, disposable, and cost-effective analytical tool for measuring FLX.
设计并表征了用于简单、快速、准确评估氟西汀（FLX）的新型经济高效的丝网印刷电位平台。该电位平台集成了FLX传感器和参比Ag/AgCl电极。这些传感器基于使用4'-硝基苯并-15-冠-5（离子载体I）、二苯并-18-冠-6（离子载体II）和2-羟丙基-β-环糊精（2-HP-β-CD）（离子载体III）作为增塑PVC基质中的中性载体。多壁碳纳米管（MWCNTs）用作亲脂性离子到电子的转换材料，四（3,5-双（三氟甲基）苯基）硼酸钠（NaTFPB）用作阴离子排除剂。所展示的平台显示出近能斯特电位响应，基于离子载体I、II和III的传感器在10 mM Tris缓冲溶液（pH 7）中的斜率分别为56.2±0.8、56.3±1.7和64.4±0.2 mV/decade，检测限分别为5.2×10、4.7×10和2.0×10 M。所有测量均在pH 7.0的10 mM Tris缓冲溶液中进行。使用反向电流计时电位法评估了MWCNTs层插入前后所展示传感器的界面电容。这些传感器用于成功测定不同药物剂型中的FLX药物。将结果与通过标准HPLC方法获得的结果进行了比较。在不同血清样品中加入固定浓度的FLX后计算回收率值。所展示的平台具有大规模生产潜力，并为测量FLX提供了一种便携式、快速、一次性且经济高效的分析工具。
