Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.
Neuroscience Center of Excellence, Louisiana State University School of Medicine, New Orleans, LA 70112, USA.
Medicina (Kaunas). 2022 May 20;58(5):681. doi: 10.3390/medicina58050681.
: The molecular mechanisms of the development of acute kidney injury (AKI) after kidney transplantation are not yet clear. The aim of this study was to confirm the genes and mechanisms related to AKI after transplantation. : To investigate potential genetic targets for AKI, an analysis of the gene expression omnibus database was used to identify key genes and pathways. After identification of differentially expressed genes, Kyoto Encyclopedia of Genes and Genome pathway enrichment analyses were performed. We identified the hub genes and established the protein-protein interaction network. : Finally, we identified 137 differentially expressed genes (59 upregulated genes and 16 downregulated genes). AKAP12, AMOT, C3AR1, LY96, PIK3AP1, PLCD4, PLCG2, TENM2, TLR2, and TSPAN5 were filtrated by the hub genes related to the development of post-transplant AKI from the Protein-Protein Interaction (PPI) network. : This may provide important evidence of the diagnostic and therapeutic biomarker of AKI.
在肾移植后发生急性肾损伤（AKI）的分子机制尚不清楚。本研究旨在确认与移植后 AKI 相关的基因和机制。
为了研究 AKI 的潜在遗传靶标，我们使用基因表达综合数据库分析来鉴定关键基因和途径。在鉴定出差异表达基因后，进行京都基因与基因组百科全书通路富集分析。我们鉴定了枢纽基因，并建立了蛋白质-蛋白质相互作用网络。
最后，我们确定了 137 个差异表达基因（59 个上调基因和 16 个下调基因）。通过蛋白质-蛋白质相互作用（PPI）网络中的枢纽基因，从 AKI 发展的相关基因中筛选出 AKAP12、AMOT、C3AR1、LY96、PIK3AP1、PLCD4、PLCG2、TENM2、TLR2 和 TSPAN5。
这可能为 AKI 的诊断和治疗生物标志物提供重要依据。
