Takaichi Minori, Osawa Kayo, Nomoto Ryohei, Nakanishi Noriko, Kameoka Masanori, Miura Makiko, Shigemura Katsumi, Kinoshita Shohiro, Kitagawa Koichi, Uda Atsushi, Miyara Takayuki, Mertaniasih Ni Made, Hadi Usman, Raharjo Dadik, Yulistiani Ratna, Fujisawa Masato, Kuntaman Kuntaman, Shirakawa Toshiro
Department of Public Health, Kobe University Graduate School of Health Sciences, Kobe 654-0142, Japan.
Department of Medical Technology, Kobe Tokiwa University, Kobe 653-0838, Japan.
Pathogens. 2022 May 4;11(5):543. doi: 10.3390/pathogens11050543.
The increase in antibiotic resistance in non-typhoidal Salmonella enterica (NTS) has been confirmed in Indonesia by this study. We confirmed the virulence genes and antimicrobial susceptibilities of clinical NTS (n = 50) isolated from chicken meat in Indonesia and also detected antimicrobial resistance genes. Of 50 strains, 30 (60%) were non-susceptible to nalidixic acid (NA) and all of them had amino acid mutations in gyrA. Among 27 tetracycline (TC) non-susceptible strains, 22 (81.5%) had tetA and/or tetB. The non-susceptibility rates to ampicillin, gentamicin or kanamycin were lower than that of NA or TC, but the prevalence of blaTEM or aadA was high. Non-susceptible strains showed a high prevalence of virulence genes compared with the susceptible strains (tcfA, p = 0.014; cdtB, p < 0.001; sfbA, p < 0.001; fimA, p = 0.002). S. Schwarzengrund was the most prevalent serotype (23 strains, 46%) and the most frequently detected as multi-antimicrobial resistant. The prevalence of virulence genes in S. Schwarzengrund was significantly higher than other serotypes in hlyE (p = 0.011) and phoP/Q (p = 0.011) in addition to the genes above. In conclusion, NTS strains isolated from Indonesian chicken had a high resistance to antibiotics and many virulence factors. In particular, S. Schwarzengrund strains were most frequently detected as multi-antimicrobial resistant and had a high prevalence of virulence genes.
本研究证实了印度尼西亚非伤寒型肠炎沙门氏菌（NTS）的抗生素耐药性增加。我们确定了从印度尼西亚鸡肉中分离出的临床NTS（n = 50）的毒力基因和抗菌药敏性，并检测了抗菌耐药基因。在50株菌株中，30株（60%）对萘啶酸（NA）不敏感，并且所有这些菌株的gyrA基因都有氨基酸突变。在27株对四环素（TC）不敏感的菌株中，22株（81.5%）有tetA和/或tetB基因。对氨苄西林、庆大霉素或卡那霉素的不敏感率低于NA或TC，但blaTEM或aadA基因的流行率较高。与敏感菌株相比，不敏感菌株的毒力基因流行率较高（tcfA，p = 0.014；cdtB，p < 0.001；sfbA，p < 0.001；fimA，p = 0.002）。施瓦岑格鲁德沙门氏菌是最常见的血清型（23株，46%），并且最常被检测为多重耐药。除上述基因外，施瓦岑格鲁德沙门氏菌中hlyE（p = 0.011）和phoP/Q（p = 0.011）毒力基因的流行率显著高于其他血清型。总之，从印度尼西亚鸡肉中分离出的NTS菌株对抗生素具有高耐药性且有许多毒力因子。特别是，施瓦岑格鲁德沙门氏菌菌株最常被检测为多重耐药且毒力基因流行率高。
