Brunello Giulia, Zanotti Federica, Trentini Martina, Zanolla Ilaria, Pishavar Elham, Favero Vittorio, Favero Riccardo, Favero Lorenzo, Bressan Eriberto, Bonora Massimo, Sivolella Stefano, Zavan Barbara
Department of Neurosciences, School of Dentistry, University of Padua, 35128 Padua, Italy.
Department of Oral Surgery, University Hospital of Düsseldorf, 40225 Dusseldorf, Germany.
Pharmaceutics. 2022 Apr 21;14(5):908. doi: 10.3390/pharmaceutics14050908.
Craniofacial tissue reconstruction still represents a challenge in regenerative medicine. Mesenchymal stem cell (MSC)-based tissue engineering strategies have been introduced to enhance bone tissue repair. However, the risk of related complications is limiting their usage. To overcome these drawbacks, exosomes (EXOs) derived from MSCs have been recently proposed as a cell-free alternative to MSCs to direct tissue regeneration. It was hypothesized that there is a correlation between the biological properties of exosomes derived from the dental pulp and the age of the donor. The aim of the study was to investigate the effect of EXOs derived from dental pulp stem cells of permanent teeth (old donor group) or exfoliated deciduous teeth (young donor group) on MSCs cultured in vitro. Proliferation potential was evaluated by doubling time, and commitment ability by gene expression and biochemical quantification for tissue-specific factors. Results showed a well-defined proliferative influence for the younger donor aged group. Similarly, a higher commitment ability was detected in the young group. In conclusion, EXOs could be employed to promote bone regeneration, likely playing an important role in neo-angiogenesis in early healing phases.
颅面组织重建在再生医学中仍然是一项挑战。基于间充质干细胞（MSC）的组织工程策略已被引入以增强骨组织修复。然而，相关并发症的风险限制了它们的应用。为克服这些缺点，最近有人提出将源自MSC的外泌体（EXO）作为MSC的无细胞替代物来指导组织再生。据推测，牙髓来源的外泌体的生物学特性与供体年龄之间存在相关性。本研究的目的是调查源自恒牙（老年供体组）或脱落乳牙（年轻供体组）的牙髓干细胞的EXO对体外培养的MSC的影响。通过倍增时间评估增殖潜力，并通过基因表达和组织特异性因子的生化定量评估分化能力。结果显示年轻供体年龄组具有明确的增殖影响。同样，在年轻组中检测到更高的分化能力。总之，EXO可用于促进骨再生，可能在早期愈合阶段的新生血管形成中发挥重要作用。
