Ouerghemmi Safa, Degoutin Stéphanie, Maton Mickael, Tabary Nicolas, Cazaux Frédéric, Neut Christel, Blanchemain Nicolas, Martel Bernard
Univ. Lille, CNRS, INRAE, Centrale Lille, UMR 8207-UMET-Unité Matériaux et Transformations, F-59000 Lille, France.
Univ. Lille, Inserm, CHU Lille, U1008, Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.
Polymers (Basel). 2022 May 11;14(10):1955. doi: 10.3390/polym14101955.
This work focuses on the manufacture of core-sheath nanofibers (NFs) based on chitosan (CHT) as sheath and cyclodextrin polymer (PCD) as core and loaded with triclosan (TCL). In parallel, monolithic NFs consisting of blended CHT-PCD and TCL were prepared. Nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier Transform Infrared spectroscopy (FTIR). SEM displayed the morphology of NFs and the structure of the nanowebs, while TEM evidenced the core-sheath structure of NFs prepared by coaxial electrospinning. The core diameters and sheath thicknesses were found dependent on respective flow rates of both precursor solutions. Nanofibers stability and TCL release in aqueous medium were studied and correlated with the antibacterial activity against and . Results showed that the release profiles of TCL and therefore the antibacterial activity were directly related to the type of nanofibers. In the case of monolithic nanofibers, the NFs matrix was composed of polyelectrolyte complex (PEC formed between CHT and PCD) and resulted in a prolonged release of TCL and a sustained antibacterial effect. In the case of core-sheath NFs, the PEC was formed only at the core-sheath interface, leading to less stable NFs and therefore to a faster release of TCL, and to a less extended antibacterial activity compared to monolithic ones.
这项工作聚焦于以壳聚糖（CHT）为鞘层、环糊精聚合物（PCD）为芯层并负载三氯生（TCL）来制造核壳纳米纤维（NFs）。同时，制备了由CHT - PCD和TCL混合而成的整体式纳米纤维。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对纳米纤维进行了表征。SEM展示了纳米纤维的形态和纳米网的结构，而TEM证实了通过同轴静电纺丝制备的纳米纤维的核壳结构。发现核直径和鞘层厚度取决于两种前驱体溶液各自的流速。研究了纳米纤维在水性介质中的稳定性和TCL释放情况，并将其与对[具体对象1]和[具体对象2]的抗菌活性相关联。结果表明，TCL的释放曲线以及因此的抗菌活性与纳米纤维的类型直接相关。对于整体式纳米纤维，纳米纤维基质由聚电解质复合物（CHT和PCD之间形成的PEC）组成，导致TCL的释放延长和持续的抗菌效果。对于核壳纳米纤维，PEC仅在核壳界面形成，导致纳米纤维稳定性较差，因此TCL释放更快，与整体式纳米纤维相比，抗菌活性的持续时间较短。
