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猪流行性腹泻病毒感染诱导自噬体形成，但在复制过程中抑制自溶酶体形成。

Porcine Epidemic Diarrhea Virus Infection Induces Autophagosome Formation but Inhibits Autolysosome Formation during Replication.

机构信息

Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon 13434, Korea.

Research Institute of Veterinary Medicine, Chungnam National University, Daejeon 13434, Korea.

出版信息

Viruses. 2022 May 15;14(5):1050. doi: 10.3390/v14051050.

DOI:10.3390/v14051050
PMID:35632790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9142955/
Abstract

In this study, we investigated the correlation between the mechanism involved in porcine epidemic diarrhea virus (PEDV) replication and autophagic flux. In this study, we found that as PEDV replicated, production of LC3-II was significantly induced up to 24 h post-infection (hpi). Interestingly, although there was significant production of LC3-II, greater p62 accumulation was simultaneously found. Pretreatment with rapamycin significantly induced PEDV replication, but autolysosome formation was reduced. These results were confirmed by the evaluation of ATG5/ATG12 and LAMP1/LAMP2. Taken together, we conclude that PEDV infection induces autophagosome formation but inhibits autolysosome formation during replication.

摘要

在这项研究中，我们调查了猪流行性腹泻病毒（PEDV）复制机制与自噬通量之间的相关性。在这项研究中，我们发现，随着 PEDV 的复制，LC3-II 的产生在感染后 24 小时内显著增加。有趣的是，尽管 LC3-II 的产生显著增加，但同时也发现 p62 的积累增加。雷帕霉素预处理显著诱导 PEDV 复制，但自噬溶酶体的形成减少。通过评估 ATG5/ATG12 和 LAMP1/LAMP2，证实了这些结果。综上所述，我们得出结论，PEDV 感染诱导自噬体形成，但在复制过程中抑制自噬溶酶体的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/4b2f92e758cc/viruses-14-01050-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/2d9c73e304f2/viruses-14-01050-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/ebf44c80d110/viruses-14-01050-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/4b2f92e758cc/viruses-14-01050-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/6a40549059a4/viruses-14-01050-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/f350d85b6c8c/viruses-14-01050-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/5d71521ea9b6/viruses-14-01050-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/40cf899918b6/viruses-14-01050-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/2d9c73e304f2/viruses-14-01050-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/ebf44c80d110/viruses-14-01050-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c042/9142955/4b2f92e758cc/viruses-14-01050-g008.jpg

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