Zhang Weixiao, Shen Haiyan, Wang Menglu, Fan Xuelei, Wang Songqi, Wuri Nile, Zhang Bin, He Haiyan, Zhang Chunhong, Liu Zhicheng, Liao Ming, Zhang Jianfeng, Li Yugu, Zhang Jianmin
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Front Microbiol. 2023 Jul 7;14:1164851. doi: 10.3389/fmicb.2023.1164851. eCollection 2023.
Animal and human health are severely threatened by coronaviruses. The enteropathogenic coronavirus, porcine epidemic diarrhea virus (PEDV), is highly contagious, leading to porcine epidemic diarrhea (PED), which causes large economic losses in the world's swine industry. Piglets are not protected from emerging PEDV variants; therefore, new antiviral measures for PED control are urgently required. Herein, the anti-PEDV effects and potential mechanisms of fangchinoline (Fan) were investigated. Fan dose-dependently inhibited a PEDV infection at 24 h post-infection (EC value = 0.67 μM). We found that Fan mainly affected the PEDV replication phase but also inhibited PEDV at the attachment and internalization stages of the viral life cycle. Mechanistically, Fan blocked the autophagic flux in PEDV-infected cells by regulating the expression of autophagy-related proteins and changing PEDV virus particles. In summary, Fan inhibits PEDV infection by blocking the autophagic flux in cells. Our findings will help develop new strategies to prevent and treat PEDV infection.
冠状病毒严重威胁着动物和人类健康。肠道致病性冠状病毒——猪流行性腹泻病毒（PEDV）具有高度传染性，可导致猪流行性腹泻（PED），给全球养猪业造成巨大经济损失。仔猪对新出现的PEDV变异株没有抵抗力；因此，迫切需要新的控制PED的抗病毒措施。在此，研究了粉防己碱（Fan）的抗PEDV作用及其潜在机制。Fan在感染后24小时剂量依赖性地抑制PEDV感染（EC值 = 0.67 μM）。我们发现Fan主要影响PEDV复制阶段，但也在病毒生命周期的附着和内化阶段抑制PEDV。机制上，Fan通过调节自噬相关蛋白的表达和改变PEDV病毒颗粒来阻断PEDV感染细胞中的自噬流。总之，Fan通过阻断细胞中的自噬流来抑制PEDV感染。我们的研究结果将有助于开发预防和治疗PEDV感染的新策略。
