State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510275, China.
Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Yunfu 527400, China.
Viruses. 2022 May 31;14(6):1196. doi: 10.3390/v14061196.
Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED) characterized by vomit, watery diarrhea, dehydration and high mortality. Outbreaks of highly pathogenic variant strains of PEDV have resulted in extreme economic losses to the swine industry all over the world. The study of host-virus interaction can help to better understand the viral pathogenicity. Many studies have shown that poly(A)-binding proteins are involved in the replication process of various viruses. Here, we found that the infection of PEDV downregulated the expression of poly(A)-binding protein cytoplasmic 1 (PABPC1) at the later infection stage in Vero cells. The overexpression of PABPC1 inhibited the proliferation of PEDV at transcription and translation level, and siRNA-mediated depletion of PABPC1 promoted the replication of PEDV. Furthermore, mass spectrometry analysis and immunoprecipitation assay confirmed that PABPC1 interacted with the nucleocapsid (N) protein of PEDV. Confocal microscopy revealed the co-localizations of PABPC1 with N protein in the cytoplasm. Taken together, these results demonstrate the antiviral effect of PABPC1 against PEDV replication by interacting with N protein, which increases understanding of the interaction between PEDV and host.
猪流行性腹泻病毒（PEDV）是引起猪流行性腹泻（PED）的病原体，其特征为呕吐、水样腹泻、脱水和高死亡率。高致病性变异株 PEDV 的爆发给全世界的养猪业造成了巨大的经济损失。宿主-病毒相互作用的研究有助于更好地了解病毒的致病性。许多研究表明，多聚（A）结合蛋白参与了各种病毒的复制过程。在这里，我们发现 PEDV 感染在 Vero 细胞中在后感染阶段下调了多聚（A）结合蛋白细胞质 1（PABPC1）的表达。PABPC1 的过表达抑制了 PEDV 在转录和翻译水平的增殖，而 siRNA 介导的 PABPC1 耗竭促进了 PEDV 的复制。此外，质谱分析和免疫沉淀实验证实 PABPC1 与 PEDV 的核衣壳（N）蛋白相互作用。共聚焦显微镜显示 PABPC1 与 N 蛋白在细胞质中共定位。综上所述，这些结果表明 PABPC1 通过与 N 蛋白相互作用对 PEDV 复制具有抗病毒作用，这增加了对 PEDV 与宿主相互作用的理解。
