WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
German Center for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel-Reims, 38124 Braunschweig, Germany.
Viruses. 2022 May 15;14(5):1052. doi: 10.3390/v14051052.
Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe disease in humans and livestock. Due to its high pathogenicity in humans and the lack of available vaccines and therapeutics, NiV needs to be handled in biosafety level 4 (BSL-4) laboratories. Safe inactivation of samples containing NiV is thus necessary to allow further processing in lower containment areas. To date, there is only limited information available on NiV inactivation methods validated by BSL-4 facilities that can be used as a reference. Here, we compare some of the most common inactivation methods in order to evaluate their efficacy at inactivating NiV in infected cells, supernatants and organs. Thus, several physical and chemical inactivation methods, and combinations thereof, were assessed. Viral replication was monitored for 3 weeks and NiV presence was assessed by RT-qPCR, plaque assay and indirect immunofluorescence. A total of nineteen methods were shown to reduce NiV infectious particles in cells, supernatants and organs to undetectable levels. Therefore, we provide a list of methods for the safe and efficient inactivation of NiV.
尼帕病毒（NiV）是一种新兴的人畜共患病副粘病毒，可导致人类和牲畜严重疾病。由于其在人类中的高致病性以及缺乏可用的疫苗和疗法，NiV 需要在生物安全 4 级（BSL-4）实验室中进行处理。因此，需要安全地灭活含有 NiV 的样本，以便在较低的控制区域进一步处理。迄今为止，只有有限的信息可用于 BSL-4 设施验证的 NiV 灭活方法，可作为参考。在这里，我们比较了一些最常见的灭活方法，以评估它们在灭活感染细胞、上清液和器官中的 NiV 的效果。因此，评估了几种物理和化学灭活方法及其组合。监测了 3 周的病毒复制，并通过 RT-qPCR、噬斑测定和间接免疫荧光评估 NiV 的存在。总共 19 种方法显示可将细胞、上清液和器官中的 NiV 感染性颗粒降低到无法检测的水平。因此，我们提供了一种安全有效地灭活 NiV 的方法列表。
