Department of Microbiology, Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari, Ehime, 794-8555, Japan.
Department of Virology I, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
Virol J. 2020 Oct 9;17(1):151. doi: 10.1186/s12985-020-01425-8.
Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could provide information on not only the diagnosis of NiV disease but also the history of an individual with previous exposure to the virus, thereby supporting disease control. Therefore, an efficient method for the inactivation of NiV in serum samples is required for serological diagnosis.
We determined the optimal conditions for the inactivation of NiV infectivity in human serum using heating and UV treatment. The inactivation method comprised UV irradiation with a cover of aluminum foil for 30 min and heating at 56 °C for 30 min.
With an optimized protocol for virus inactivation, NiV infectivity in serum samples (containing 6.0 × 10 TCID) was completely inactivated.
We developed a recommended protocol for the effective inactivation of NiV. This protocol would enable a regional or local laboratory to safely transport or process samples, including NiV, for serological testing in its biosafety level-2 facility.
尼帕病毒（NiV）是一种新兴的人畜共患副粘病毒，可导致人类严重脑炎和呼吸道疾病，死亡率很高。在病毒性疾病的大爆发期间，血清样本的血清学检测可能是一种有用的诊断工具，不仅可以提供 NiV 疾病诊断的信息，还可以提供个体以前接触过该病毒的历史信息，从而支持疾病控制。因此，需要一种有效的方法来灭活血清样本中的尼帕病毒，以进行血清学诊断。
我们使用加热和 UV 处理来确定在人血清中灭活 NiV 感染性的最佳条件。该灭活方法包括用铝箔覆盖 30 分钟的 UV 照射和 56°C 加热 30 分钟。
通过优化的病毒灭活方案，血清样本中的 NiV 感染力（含 6.0×10 TCID）完全被灭活。
我们开发了一种推荐的 NiV 有效灭活方案。该方案将使地区或当地实验室能够在其生物安全二级设施中安全地运输或处理包括 NiV 在内的样本，以进行血清学检测。
