College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Taian City Central Hospital, Taian, 271000, China.
Virus Genes. 2022 Oct;58(5):458-466. doi: 10.1007/s11262-022-01917-5. Epub 2022 May 28.
Stenotrophomonas maltophilia has been recognized as an emerging global opportunistic pathogen, and it is intrinsically resistant to most antibiotics, which makes the limited choice for treating S. maltophilia infections. Bacteriophage with the proper characterization is considered as a promising alternative treatment option to control S. maltophilia infections. In this study, we isolated a novel Siphoviridae bacteriophage vB_SmaS_BUCT626 with lytic activity against S. maltophilia. Phage vB_SmaS_BUCT626 can lysis 10 of 20 S. maltophilia and was relatively stable at a wide range of temperatures (4-70 °C) and pH values (3.0-13.0) and exhibited good tolerance to chloroform. The genome of phage vB_SmaS_BUCT626 was a 61,662-bp linear double-stranded DNA molecule with a GC content of 56.2%, and contained 100 open-reading frames. It carried no antibiotic resistance, toxin, virulence-related genes, or lysogen-formation gene clusters. Together, these characteristics make phage vB_SmaS_BUCT626, a viable candidate as a biocontrol agent against S. maltophilia infection.
嗜麦芽寡养单胞菌已被确认为一种新出现的全球机会性病原体，其对大多数抗生素具有固有耐药性，这使得治疗嗜麦芽寡养单胞菌感染的选择有限。具有适当特征的噬菌体被认为是控制嗜麦芽寡养单胞菌感染的一种有前途的替代治疗选择。在本研究中，我们分离到了一种新型的 Siphoviridae 噬菌体 vB_SmaS_BUCT626，对嗜麦芽寡养单胞菌具有裂解活性。噬菌体 vB_SmaS_BUCT626 可以裂解 20 株嗜麦芽寡养单胞菌中的 10 株，并且在很宽的温度（4-70°C）和 pH 值（3.0-13.0）范围内相对稳定，并且对氯仿具有良好的耐受性。噬菌体 vB_SmaS_BUCT626 的基因组是一个 61662 碱基对的线性双链 DNA 分子，GC 含量为 56.2%，包含 100 个开放阅读框。它不携带抗生素抗性、毒素、毒力相关基因或溶原形成基因簇。综上所述，噬菌体 vB_SmaS_BUCT626 作为一种控制嗜麦芽寡养单胞菌感染的生物防治剂具有很大的潜力。
