肠易激综合征的肠道微生物群与严重程度的相关性。
Correlation between Intestinal Microflora in Irritable Bowel Syndrome and Severity.
机构信息
The Second People's Hospital of Longgang District, Shenzhen, Guangdong 518112, China.
出版信息
Dis Markers. 2022 May 18;2022:1031844. doi: 10.1155/2022/1031844. eCollection 2022.
BACKGROUND
Irritable bowel syndrome (IBS) is a common chronic functional gastrointestinal disease accompanied by changes in intestinal microecology. This study investigated the relationship between gut microbiota and disease severity in patients with irritable bowel syndrome (IBS).
METHODS
An observational study was performed on 60 IBS patients (study group) and 20 healthy controls admitted to our hospital from January 2013 to December 2014. Fecal samples were taken after admission to measure intestinal flora including Bifidobacterium, Lactobacillus, Enterobacter, and Enterococcus, and patient blood was collected to determine serum D-lactate and diamine oxidase (DAO) levels. The gut microbiota and serum markers of the two groups were analyzed. The correlation of gut microbiota index levels and serum markers with disease severity, as well as the correlation between gut microbiota index levels and serum markers, were analyzed.
RESULTS
The levels of intestinal and were lower, while the levels of and and serum D-lactate were higher in the study group than those in the control group. The levels of intestinal and were lower, while the levels of and , serum D-lactate, and DAO were higher in patients with moderate IBS than those in patients with mild IBS. The levels of intestinal and were lower in patients with severe IBS than those with moderate IBS, while the levels of and , serum D-lactate, and DAO were higher in patients with severe IBS. There was a significant negative correlation between the levels of and and disease severity and a significant positive correlation between the levels of and , D-lactate, and DAO and disease severity. There was a significant negative correlation between the levels of and and serum D-lactate and DAO, while there was a significant positive correlation between the levels of and and serum D-lactate and DAO ( < 0.05).
CONCLUSION
Intestinal flora, D-lactate, and DAO were abnormal in IBS patients, and intestinal flora was closely correlated with disease severity, D-lactate, and DAO levels.
背景
肠易激综合征（IBS）是一种常见的慢性功能性胃肠道疾病，伴有肠道微生态的变化。本研究旨在探讨肠易激综合征（IBS）患者肠道微生物群与疾病严重程度的关系。
方法
采用观察性研究方法，选取 2013 年 1 月至 2014 年 12 月我院收治的 60 例 IBS 患者（研究组）和 20 例健康对照者（对照组）。入院后采集粪便标本，检测双歧杆菌、乳杆菌、肠杆菌和肠球菌等肠道菌群，采集患者血液，检测血清 D-乳酸和二胺氧化酶（DAO）水平。分析两组患者的肠道菌群和血清标志物。分析肠道菌群指标水平与血清标志物与疾病严重程度的相关性，以及肠道菌群指标水平与血清标志物的相关性。
结果
研究组患者肠道双歧杆菌和乳杆菌水平较低，肠杆菌和肠球菌水平以及血清 D-乳酸水平较高。中度 IBS 患者肠道双歧杆菌和乳杆菌水平较低，肠杆菌和肠球菌水平、血清 D-乳酸、DAO 水平较高。重度 IBS 患者肠道双歧杆菌和乳杆菌水平较中度 IBS 患者更低，肠杆菌和肠球菌水平、血清 D-乳酸、DAO 水平更高。患者肠道双歧杆菌和乳杆菌水平与疾病严重程度呈显著负相关，肠杆菌和肠球菌水平、血清 D-乳酸、DAO 水平与疾病严重程度呈显著正相关。患者肠道双歧杆菌和乳杆菌水平与血清 D-乳酸、DAO 水平呈显著负相关，肠杆菌和肠球菌水平与血清 D-乳酸、DAO 水平呈显著正相关（<0.05）。
结论
IBS 患者肠道菌群、D-乳酸和 DAO 异常，肠道菌群与疾病严重程度、D-乳酸和 DAO 水平密切相关。
Zotero 插件