Large Animal Clinical Sciences, University of Florida College of Veterinary Medicine, Gainesville, Florida, USA.
J Vet Intern Med. 2022 Sep;36(5):1812-1819. doi: 10.1111/jvim.16450. Epub 2022 May 31.
Atlantoaxial (AA) cerebrospinal fluid (CSF) collection in standing horses utilizes the controlled narcotic morphine, thereby limiting feasibility in field practice settings.
To compare AA CSF collection time and reaction scores in horses sedated with morphine-containing and opioid-free sedation protocols: detomidine + morphine (DM), detomidine + xylazine (DX), detomidine + detomidine (DD), detomidine alone (D0); To develop a novel method for assessing sedation in standing horses using open-source motion-tracking software.
Six healthy adult horses.
Randomized crossover. Atlantoaxial CSF collections were performed weekly for 4 weeks. Horses received sedation protocols in random order. Procedure time and procedure reaction scores were compared between groups using Friedman test. Associations between procedure reaction scores and motion tracking variables (total distance and farthest excursion traveled by horse's head) were examined using scatter diagrams and linear regression.
Procedure times were lower in horses sedated with DX (median: 36 seconds; range: 28 - 188), compared to D0 (121; 35 - 196; P = 0.04). Procedure reaction scores were lower in horses sedated with DX (median 1.0, range 1.0 to 2.0), or DM (1.8; 1.3 - 3.0) compared to DO (3.0; 2.3 - 3.0; P = .03). Reactions to dura mater puncture were recorded in 3 of 6 horses in D0 and DD groups, and 0 of 6 horses in DX and DM groups. Positive associations were observed between reaction score vs total distance or farthest excursion distance from baseline.
Both opioid-free and morphine-containing sedation protocols are acceptable for AA CSF collection. Motion-tracking software represents a novel method for assessing sedation in standing horses.
在站立的马中进行寰枢（AA）脑脊液（CSF）采集时使用了受控制的麻醉性吗啡，因此限制了在野外实践环境中的可行性。
比较使用含吗啡和不含阿片类药物镇静方案镇静的马匹的 AA CSF 采集时间和反应评分：含吗啡的地西泮（DM）、含二甲噻嗪的地西泮（DX）、含地西泮的地西泮（DD）、单独使用地西泮（D0）；开发一种使用开源运动跟踪软件评估站立马匹镇静的新方法。
六匹健康成年马。
随机交叉。每周进行 4 周的 AA CSF 采集。马匹按随机顺序接受镇静方案。使用 Friedman 检验比较组间程序时间和程序反应评分。使用散点图和线性回归检查程序反应评分与运动跟踪变量（马头部的总距离和最远偏移量）之间的关联。
与 D0 相比，DX 镇静的马匹（中位数：36 秒；范围：28-188）的程序时间较低（P=0.04）。DX（中位数 1.0，范围 1.0-2.0）或 DM（1.8；1.3-3.0）镇静的马匹的程序反应评分低于 D0（3.0；2.3-3.0；P=0.03）。D0 和 DD 组的 3 匹马和 D0 组的 6 匹马中记录到对硬脑膜穿刺的反应。反应评分与总距离或与基线的最远偏移距离呈正相关。
两种无阿片类药物和含吗啡的镇静方案均可用于 AA CSF 采集。运动跟踪软件代表了评估站立马匹镇静的一种新方法。
