Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Guangxi Key Lab of Sugarcane Biology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China.
Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Plant Sci. 2022 Jul;320:111284. doi: 10.1016/j.plantsci.2022.111284. Epub 2022 Apr 14.
Plant-specific calcineurin B-like proteins (CBLs) and their interacting kinases, CBL-interacting protein kinases (CIPKs) module, are essential for dealing with various biotic and abiotic stress. The kinases (CIPKs) of this module have been well studied in several plants; however, the information about pineapple CIPKs remains limited. To understand how CIPKs function against environmental cues in pineapple, the CIPK5 gene of pineapple was cloned and characterized. The phylogenetic analyses revealed that AcCIPK5 is homologous to the CIPK12 of Arabidopsis and other plant species. Quantitative real-time PCR (qRT-PCR) analysis revealed that AcCIPK5 responds to multiple stresses, including osmotic, salt stress, heat and cold. Under optimal conditions, AcCIPK5 gets localized to the cytoplasm and cell membrane. The ectopic expression of AcCIPK5 in Arabidopsis improved the germination under osmotic and salt stress. Furthermore, AcCIPK5 positively regulated osmotic, drought, salt and cold tolerance and negatively regulated heat and fungal stress in Arabidopsis. Besides, the expression of AcCIPK impacted ABA-related genes and ROS homeostasis. Overall, the present study demonstrates that AcCIPK5 contributes to multiple stress tolerance and has the potential to be utilized in the development of stress-tolerant crops.
植物特异性钙调神经磷酸酶 B 类似蛋白（CBL）及其相互作用的激酶，CBL 相互作用蛋白激酶（CIPK）模块，对于应对各种生物和非生物胁迫至关重要。该模块中的激酶（CIPKs）在几种植物中得到了很好的研究；然而，菠萝 CIPKs 的信息仍然有限。为了了解 CIPKs 如何针对菠萝中的环境线索发挥作用，克隆并表征了菠萝的 CIPK5 基因。系统发育分析表明，AcCIPK5 与拟南芥和其他植物物种的 CIPK12 同源。定量实时 PCR（qRT-PCR）分析显示，AcCIPK5 对多种胁迫（包括渗透、盐胁迫、热和冷）有反应。在最佳条件下，AcCIPK5 定位于细胞质和细胞膜。在拟南芥中异位表达 AcCIPK5 可提高其在渗透和盐胁迫下的萌发率。此外，AcCIPK5 正向调控拟南芥的渗透、干旱、盐和冷胁迫耐受性，负向调控热和真菌胁迫耐受性。此外，AcCIPK 的表达影响 ABA 相关基因和 ROS 稳态。总的来说，本研究表明 AcCIPK5 有助于多种胁迫耐受性，并有潜力被用于开发抗胁迫作物。
