Lin Haisheng, Shen Weiqiang, Jiang Yu, Wu Qihang, Gao Jialong, Cao Wenhong, Zheng Huina, Chen Zhongqin, Zhong Saiyi, Qin Xiaoming
Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China.
Foods. 2024 Mar 14;13(6):883. doi: 10.3390/foods13060883.
In our previous study, two peptides with favorable anti-inflammatory effects, Asp-Gln-Thr-Phe (DQTF) and Gly-Tyr-Thr-Arg (GYTR), were screened from using an in vitro-in silico strategy. The present study aims to investigate the ameliorative effect of peptides (RPPs) on acute inflammation and clarify the potential mechanism through in vitro and in vivo experiments. The anti-inflammatory effects of DQTF and GYTR were verified with a lipopolysaccharide (LPS)-induced RAW264.7 cell acute inflammation model and the anti-inflammatory effect of the enzymatic hydrolysates of was explored in vivo using an LPS-induced acute inflammatory injury model in mice. The results show that DQTF and GYTR improved the morphology of LPS-injured cells and decreased the concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in LPS-induced cells. Moreover, the antioxidant enzyme activity in cells was markedly increased with DQTF and GYTR. The enzymatic hydrolysates of were obtained with hydrolysis using pepsin-chymotrypsin-trypsin (PeCTHC) and pepsin-trypsin (PeTHC), respectively. PeCTHC and PeTHC significantly reduced pro-inflammatory cytokines and nitric oxide (NO) in the serum. Additionally, the blood indices and levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) in the livers of mice were markedly improved with RPPs administration. In conclusion, RPPs have preventive and protective effects on acute inflammation, with significant prospects for development in the field of functional foods.
在我们之前的研究中，使用体外-计算机模拟策略从[具体物质]中筛选出了两种具有良好抗炎作用的肽，天冬酰胺-谷氨酰胺-苏氨酸-苯丙氨酸（DQTF）和甘氨酸-酪氨酸-苏氨酸-精氨酸（GYTR）。本研究旨在通过体外和体内实验研究[具体肽类物质]（RPPs）对急性炎症的改善作用，并阐明其潜在机制。采用脂多糖（LPS）诱导的RAW264.7细胞急性炎症模型验证了DQTF和GYTR的抗炎作用，并使用LPS诱导的小鼠急性炎症损伤模型在体内探索了[具体物质]酶解产物的抗炎作用。结果表明，DQTF和GYTR改善了LPS损伤细胞的形态，降低了LPS诱导细胞中肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的浓度。此外，DQTF和GYTR使细胞中的抗氧化酶活性显著增加。分别使用胃蛋白酶-糜蛋白酶-胰蛋白酶（PeCTHC）和胃蛋白酶-胰蛋白酶（PeTHC）水解得到了[具体物质]的酶解产物。PeCTHC和PeTHC显著降低了血清中的促炎细胞因子和一氧化氮（NO）。此外，给予RPPs后，小鼠肝脏的血液指标以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）和丙二醛（MDA）水平均得到显著改善。总之，RPPs对急性炎症具有预防和保护作用，在功能性食品领域具有显著的开发前景。
