大鼠坐骨神经扩散张量成像中单激发回波平面成像与读出分段回波平面成像的性能比较
Performance of Single-Shot Echo-Planar Imaging in Diffusion Tensor Imaging in Rat Sciatic Nerve Compared With Readout-Segmented Echo-Planar Imaging.
作者信息
Chen Yueyao, Pan Zhongxian, Meng Fanqi, Li Zhujing, Hu Yuanming, Yu Xuewen, Gao Jinyun, Guo Yihao, Lyu Hanqing, Lin Xiaofeng
机构信息
Department of Radiology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, China.
Department of Pathology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, China.
出版信息
Front Neurosci. 2022 May 12;16:844408. doi: 10.3389/fnins.2022.844408. eCollection 2022.
OBJECTIVES
To compare the performances of single-shot echo-planar imaging (SS-EPI) and readout-segmented echo-planar imaging (RS-EPI) for diffusion tensor imaging (DTI) of the rat sciatic nerve.
METHODS
Eight healthy adult male Sprague-Dawley rats were anesthetized and scanned with a 3T MRI scanner using SS-EPI and RS-EPI DTI sequences. The image quality in terms of the morphology of the nerve, distortions of the nearby femur, muscles, and homogeneity of neuromuscular were evaluated and scored. The correlations between the DTI parameters including fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD), apparent diffusion coefficient (ADC), and histopathological parameters were calculated by using the Pearson correlation coefficient and compared by the modified Fisher -transform, respectively.
RESULTS
The quality scores were higher for the images from the SS-EPI sequence compared with the RS-EPI sequence for characteristics such as sharpness of the sciatic nerve margin ( = 0.008), artifacts of the sciatic nerve ( = 0.008), and homogeneity of the neuromuscular region ( = 0.007), as well as the contrast-to-noise ratio (CNR) of DW images ( < 0.001). The correlation coefficients were higher for the FA and RD values from the SS-EPI sequence compared with those from the RS-EPI sequence. Furthermore, the correlation coefficients between FA and myelin thickness ( = 0.027), FA and diameter of the myelinated fiber ( = 0.036), as well as RD and myelin thickness ( = 0.05) were statistically higher for the SS-EPI sequence compared with those for the RS-EPI sequence.
CONCLUSION
Diffusion tensor imaging analysis of the rat sciatic nerve showed that the image quality from the SS-EPI sequence was significantly higher compared with that from the RS-EPI sequence. Furthermore, the FA and RD derived from the SS-EPI sequence are promising and sensitive biomarkers to detect the histopathological changes in the rat sciatic nerve.
目的
比较单次激发回波平面成像（SS-EPI）和读出分段回波平面成像（RS-EPI）在大鼠坐骨神经扩散张量成像（DTI）中的表现。
方法
对8只健康成年雄性Sprague-Dawley大鼠进行麻醉，使用3T MRI扫描仪采用SS-EPI和RS-EPI DTI序列进行扫描。从神经形态、附近股骨和肌肉的变形以及神经肌肉的均匀性方面评估图像质量并评分。使用Pearson相关系数计算包括分数各向异性（FA）、轴向扩散率（AD）、径向扩散率（RD）、表观扩散系数（ADC）在内的DTI参数与组织病理学参数之间的相关性，并分别通过修正的Fisher变换进行比较。
结果
与RS-EPI序列相比，SS-EPI序列图像在坐骨神经边缘清晰度（=0.008）、坐骨神经伪影（=0.008）、神经肌肉区域均匀性（=0.007）以及DW图像的对比噪声比（CNR）（<0.001）等特征方面质量得分更高。与RS-EPI序列相比，SS-EPI序列的FA和RD值的相关系数更高。此外，与RS-EPI序列相比，SS-EPI序列的FA与髓鞘厚度（=0.027）、FA与有髓纤维直径（=0.036）以及RD与髓鞘厚度（=0.05）之间的相关系数在统计学上更高。
结论
大鼠坐骨神经的扩散张量成像分析表明，与RS-EPI序列相比，SS-EPI序列的图像质量显著更高。此外，源自SS-EPI序列的FA和RD是检测大鼠坐骨神经组织病理学变化的有前景且敏感的生物标志物。
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