College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
PeerJ. 2022 May 23;10:e13497. doi: 10.7717/peerj.13497. eCollection 2022.
Aflatoxin B1 (AFB1) is a secondary metabolite produced by the fungus Aspergillus, which is ubiquitous in moldy grain products. Aflatoxin B1 has been reported to possess hepatotoxicity, renal toxicity, and reproductive toxicity. Previous studies have shown that AFB1 is toxic to mammalian oocytes. However, the potential toxicity of AFB1 on the organelles of mouse oocytes is unknown. In this study, we found that exposure to AFB1 significantly reduced mouse oocyte development capacity. Further analysis showed that the endoplasmic reticulum (ER) failed to accumulate around the spindle, and scattered in the cytoplasm under AFB1 exposure. Similar to the ER, the Golgi apparatus showed a uniform localization pattern following AFB1 treatment. In addition, we found that AFB1 exposure caused the condensation of lysosomes in the cytoplasm, presenting as a clustered or spindle peripheral-localization pattern, which indicated that protein modification, transport, and degradation were affected. Mitochondrial distribution was also altered by AFB1 treatment. In summary, our study showed that AFB1 exposure had toxic effects on the distribution of mouse oocyte organelles, which further led to a decline in oocyte quality.
黄曲霉毒素 B1（AFB1）是一种由真菌 Aspergillus 产生的次级代谢产物，普遍存在于霉变谷物产品中。已有报道称，黄曲霉毒素 B1 具有肝毒性、肾毒性和生殖毒性。先前的研究表明，AFB1 对哺乳动物卵母细胞有毒性。然而，AFB1 对小鼠卵母细胞细胞器的潜在毒性尚不清楚。在这项研究中，我们发现暴露于 AFB1 显著降低了小鼠卵母细胞的发育能力。进一步分析表明，内质网（ER）在 AFB1 暴露下未能在纺锤体周围聚集，而是散在细胞质中。与 ER 相似，高尔基体在 AFB1 处理后表现出均匀的定位模式。此外，我们发现 AFB1 暴露导致细胞质中溶酶体的凝聚，呈现出聚集或纺锤体周边定位模式，这表明蛋白质修饰、运输和降解受到影响。线粒体的分布也受到 AFB1 处理的影响。总之，我们的研究表明，AFB1 暴露对小鼠卵母细胞细胞器的分布具有毒性作用，进一步导致卵母细胞质量下降。
