Chen Ting, Zhuang Bo, Huang Yueqi, Liu Yan, Yuan Bochuan, Wang Wanmei, Yuan Tianyu, Du Lina, Jin Yiguang
Guangdong Pharmaceutical University, Guangzhou 510006, China.
Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China.
Acta Pharm Sin B. 2022 May;12(5):2522-2532. doi: 10.1016/j.apsb.2021.10.027. Epub 2021 Nov 5.
Radiation therapy is an effective method to kill cancer cells and shrink tumors using high-energy X-ray or -ray. Radiation pneumonitis (RP) is one of the most serious complications of radiation therapy for thoracic cancers, commonly leading to serious respiratory distress and poor prognosis. Here, we prepared curcumin-loaded mesoporous polydopamine nanoparticles (CMPN) for prevention and treatment of RP by pulmonary delivery. Mesoporous polydopamine nanoparticles (MPDA) were successfully synthesized with an emulsion-induced interface polymerization method and curcumin was loaded in MPDA stacking and hydrogen bonding interaction. MPDA owned the uniform spherical morphology with numerous mesopores that disappeared after loading curcumin. More than 80% curcumin released from CMPN in 6 h and mesopores recovered. CMPN remarkably protected BEAS-2B cells from -ray radiation injury by inhibiting apoptosis. RP rat models were established after a single dose of 15 Gy Co -ray radiation was performed on the chest area. Effective therapy of RP was achieved by intratracheal administration of CMPN due to free radical scavenging and anti-oxidation ability, and reduced proinflammatory cytokines, high superoxide dismutase, decreased malondialdehyde, and alleviated lung tissue damages were observed. Inhaled CMPN paves a new avenue for the treatment of RP.
放射治疗是一种利用高能X射线或γ射线杀死癌细胞并缩小肿瘤的有效方法。放射性肺炎（RP）是胸部癌症放射治疗最严重的并发症之一，通常会导致严重的呼吸窘迫和预后不良。在此，我们制备了负载姜黄素的介孔聚多巴胺纳米颗粒（CMPN），用于通过肺部给药预防和治疗RP。采用乳液诱导界面聚合法成功合成了介孔聚多巴胺纳米颗粒（MPDA），并通过堆积和氢键相互作用将姜黄素负载到MPDA中。MPDA具有均匀的球形形态，带有许多介孔，在负载姜黄素后介孔消失。超过80%的姜黄素在6小时内从CMPN中释放出来，介孔得以恢复。CMPN通过抑制细胞凋亡显著保护BEAS-2B细胞免受γ射线辐射损伤。在胸部区域单次给予15 Gy钴γ射线辐射后建立RP大鼠模型。由于具有自由基清除和抗氧化能力，通过气管内给予CMPN实现了对RP的有效治疗，观察到促炎细胞因子减少、超氧化物歧化酶升高、丙二醛降低以及肺组织损伤减轻。吸入CMPN为RP的治疗开辟了一条新途径。
