阿拉伯胶补充剂对小鼠单核细胞来源的巨噬细胞中抗菌肽表达的影响。
The effect of gum Arabic supplementation on cathelicidin expression in monocyte derived macrophages in mice.
机构信息
Department of Physiology, Faculty of Medicine, Red Sea University, Portsudan, Sudan.
Physiology Department, College of Medicine, King Khalid University, Abha, Saudi Arabia.
出版信息
BMC Complement Med Ther. 2022 Jun 1;22(1):149. doi: 10.1186/s12906-022-03627-9.
BACKGROUND
Antimicrobial peptides (AMPs) are important effectors of the innate defense system. Cathelicidins, (CRAMP in mouse/rat, LL-37 in human) is one of the two major classes of AMPs in humans. The upregulation of LL-37 synthesis is a novel non-antibiotic approach to prevent or treat infectious diseases. Butyrate was found to induce Cathelicidin expression. Gum Arabic (GA), an exudate from Acacia senegaltree, is known for its prebiotic effects. Fermentation of GA by colonic bacteria increases serum butyrate concentrations. This study was conducted to investigate if GA supplementation can increase Cathelicidin expression in macrophages.
METHODS
The study was an in-vivo experiment in mice. Thirty mice were randomly divided into three groups, ten mice per group. The two intervention groups received GA dissolved in drinking water in two different concentrations (15% w/v and 30% w/v) for 28 days. The third group served as a control. Blood was collected on Day 29 to isolate peripheral blood mononuclear cells (PBMC) which were cultured to obtain monocyte derived macrophages (MDMs). The transcription level of CRAMP was determined in MDMsby qPCR.
RESULTS
We detected a significant increase (p = 0.023) in CRAMP expression in MDMs following 28 days of 15% GA supplementation, compared to the control group, but there was no significant change in the group on 30% GA supplementation (p = 0.055).
CONCLUSION
GAsupplementation can induce Cathelicidin expression in MDMs and the effect is dose dependent.
背景
抗菌肽 (AMPs) 是先天防御系统的重要效应物。 抗菌肽分为两类，其中 cathelicidins（在鼠/大鼠中为 CRAMP，在人中为 LL-37）是人类中主要的 AMP 之一。上调 LL-37 的合成是预防或治疗感染性疾病的一种新的非抗生素方法。 已发现丁酸盐可诱导 Cathelicidin 的表达。 阿拉伯树胶 (GA) 是金合欢树的渗出物，以其益生元作用而闻名。 结肠细菌对 GA 的发酵会增加血清丁酸盐浓度。本研究旨在探讨 GA 补充是否可以增加巨噬细胞中 Cathelicidin 的表达。
方法
本研究是一项在小鼠体内进行的实验。 30 只小鼠被随机分为三组，每组 10 只。 两个干预组分别以两种不同浓度（15%w/v 和 30%w/v）的 GA 溶解在饮用水中连续 28 天。 第三组作为对照组。第 29 天采集血液以分离外周血单核细胞（PBMC），然后将其培养以获得单核细胞衍生的巨噬细胞（MDM）。通过 qPCR 测定 MDM 中 CRAMP 的转录水平。
结果
与对照组相比，15%GA 补充 28 天后，MDM 中 CRAMP 的表达显著增加（p=0.023），但在 30%GA 补充组中没有明显变化（p=0.055）。
结论
GA 补充可诱导 MDM 中 Cathelicidin 的表达，且呈剂量依赖性。
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