Chen Xiaoying, Xie Wei, Zhang Ming, Shi Yuhan, Xu Shaofen, Cheng Haoyu, Wu Lihong, Pathak Janak L, Zheng Zhichao
Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China.
Department of Basic Oral Medicine, School and Hospital of Stomatology, Guangzhou Medical University, Guangzhou, China.
Front Cell Dev Biol. 2022 May 16;10:903278. doi: 10.3389/fcell.2022.903278. eCollection 2022.
Autologous bone marrow-derived mesenchymal stem cells (BMSCs) are more easily available and frequently used for bone regeneration in clinics. Osteogenic differentiation of BMSCs involves complex regulatory networks affecting bone formation phenomena. Non-coding RNAs (ncRNAs) refer to RNAs that do not encode proteins, mainly including microRNAs, long non-coding RNAs, circular RNAs, piwi-interacting RNAs, transfer RNA-derived small RNAs, etc. Recent and studies had revealed the regulatory role of ncRNAs in osteogenic differentiation of BMSCs. NcRNAs had both stimulatory and inhibitory effects on osteogenic differentiation of BMSCs. During the physiological condition, osteo-stimulatory ncRNAs are upregulated and osteo-inhibitory ncRNAs are downregulated. The opposite effects might occur during bone degenerative disease conditions. Intracellular ncRNAs and ncRNAs from neighboring cells delivered via exosomes participate in the regulatory process of osteogenic differentiation of BMSCs. In this review, we summarize the recent advances in the regulatory role of ncRNAs on osteogenic differentiation of BMSCs during physiological and pathological conditions. We also discuss the prospects of the application of modulation of ncRNAs function in BMSCs to promote bone tissue regeneration in clinics.
自体骨髓间充质干细胞（BMSCs）更容易获取，在临床上常用于骨再生。BMSCs的成骨分化涉及影响骨形成现象的复杂调控网络。非编码RNA（ncRNAs）是指不编码蛋白质的RNA，主要包括微小RNA、长链非编码RNA、环状RNA、piwi相互作用RNA、转运RNA衍生的小RNA等。最近的研究揭示了ncRNAs在BMSCs成骨分化中的调控作用。ncRNAs对BMSCs的成骨分化既有刺激作用，也有抑制作用。在生理条件下，促进成骨的ncRNAs上调，抑制成骨的ncRNAs下调。在骨退行性疾病状态下可能会出现相反的效果。细胞内的ncRNAs和通过外泌体传递的来自邻近细胞的ncRNAs参与了BMSCs成骨分化的调控过程。在这篇综述中，我们总结了ncRNAs在生理和病理条件下对BMSCs成骨分化调控作用的最新进展。我们还讨论了调节BMSCs中ncRNAs功能以促进临床骨组织再生的应用前景。
