Aw T Y, Andersson B S, Jones D P
Am J Physiol. 1987 Apr;252(4 Pt 1):C356-61. doi: 10.1152/ajpcell.1987.252.4.C356.
The distribution of pyruvate, phosphate, malate, citrate, K+, aspartate, glutamate, ADP, and ATP between the mitochondrial and cytosolic compartments was studied in isolated rat hepatocytes exposed to 30 min anoxia. The results show that pyruvate and citrate gradients are comparable to aerobic values, indicating that the pH gradient across the membrane under anaerobic conditions is comparable to that under normal aerobic conditions. In contrast, the distribution of phosphate, malate, ATP, ADP, aspartate, and glutamate suggests that transport of these species may be inhibited during anoxia. The results are discussed in terms of potential regulation of mitochondrial function to provide a quiescent anoxic state that is capable of recovering normal function on reoxygenation.
在暴露于30分钟缺氧环境的分离大鼠肝细胞中，研究了丙酮酸、磷酸盐、苹果酸、柠檬酸、钾离子、天冬氨酸、谷氨酸、二磷酸腺苷（ADP）和三磷酸腺苷（ATP）在线粒体和胞质区室之间的分布。结果表明，丙酮酸和柠檬酸梯度与有氧状态下的值相当，这表明厌氧条件下跨膜的pH梯度与正常有氧条件下的相当。相比之下，磷酸盐、苹果酸、ATP、ADP、天冬氨酸和谷氨酸的分布表明，在缺氧期间这些物质的转运可能受到抑制。根据线粒体功能的潜在调节对结果进行了讨论，以提供一种静止的缺氧状态，该状态能够在复氧后恢复正常功能。
