Anderson Stephanie, Barrantes Murillo Daniel Felipe, Womble Mandy, Gibbs Nicole, Harrell Karyn, Negrão Watanabe Tatiane Terumi
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
Front Vet Sci. 2022 May 17;9:878327. doi: 10.3389/fvets.2022.878327. eCollection 2022.
A 2.5-year-old, 25.5 kg, spayed female Australian Shepherd dog had a 2-month history of shifting leg lameness in all limbs, tetraparesis, progressive lethargy, and severe pain. On the physical examination, fever (40.61°C), tachycardia, tachypnea, mild diffuse pelvic limb muscular atrophy, left prescapular and right popliteal lymphadenomegaly were observed. Due to the poor prognosis and difficult pain management, humane euthanasia was elected. Macroscopic and histological findings revealed multifocal to coalescing granulomas with central areas of lytic necrosis within the right femur, left humerus, left scapula, left biceps brachii, right semimembranosus muscle, liver, spleen, and lymph nodes. The necrotic areas contained myriad intralesional, intracellular, and extracellular negatively stained, non-pigmented, septate acute angle branching hyphae with parallel walls measuring 3-6 μm in width with polar bulbous projections measuring 7-13 μm in width. Fresh samples of the liver were submitted for fungal culture. Panfungal PCR targeting the major conserved genes-ITS, TUB, CAL-confirmed spp. are members of anamorphic fungi classified under the phylum Ascomycota. Paecilomycosis is an uncommon fungal infection caused by spp with a disease reported in humans and animals ranging from superficial to systemic clinical forms affecting both immunocompromised and immunocompetent individuals. In dogs, disseminated paecilomycosis has been reported, but the species of fungi are not always determined. To our knowledge, this is the first case of disseminated paecilomycosis caused by infection in a dog.
一只2.5岁、体重25.5千克的已绝育雌性澳大利亚牧羊犬，有两个月来四肢交替性跛行、四肢轻瘫、进行性嗜睡和剧痛的病史。体格检查发现发热（40.61°C）、心动过速、呼吸急促、轻度弥漫性骨盆肢体肌肉萎缩、左肩胛前和右腘淋巴结肿大。由于预后不良且疼痛管理困难，选择了安乐死。大体和组织学检查结果显示，在右股骨、左肱骨、左肩胛骨、左肱二头肌、右半膜肌、肝脏、脾脏和淋巴结内有多灶性至融合性肉芽肿，中央有溶解性坏死区域。坏死区域含有无数病灶内、细胞内和细胞外的负染、无色素、有隔、呈锐角分支的菌丝，其平行壁宽3 - 6μm，极部球茎状突起宽7 - 13μm。采集肝脏新鲜样本进行真菌培养。针对主要保守基因ITS、TUB、CAL的泛真菌PCR证实该菌属于子囊菌门下分类的无性型真菌。拟青霉病是一种由该菌引起的罕见真菌感染，在人和动物中均有报道，其疾病范围从浅表到全身临床形式，影响免疫功能低下和免疫功能正常的个体。在犬类中，已报道有播散性拟青霉病，但真菌种类并不总是能确定。据我们所知，这是首例由该菌感染引起的犬播散性拟青霉病病例。
